We assessed a new screening method, based on -hemolysin production in the presence of 6 mg/liter vancomycin, to distinguishheteroresistant vancomycin-intermediate Staphylococcus aureus (hVISA) and vancomycin-intermediate S. aureus (VISA) fromvancomycin-susceptible S. aureus (VSSA). On 37 clinical methicillin-resistant S. aureus (MRSA) isolates, hVISA and VISA displayedno -hemolysis whereas VSSA displayed strong -hemolysis, showing 91.6% sensitivity. These data, supported by realtimereverse transcription PCR (real-time RT-PCR) highlighting an hld downregulation, i.e., VSSA>hVISA>VISA, define thisnew assay as a valid screening method.
A novel delta-hemolysis screening method for detecting heteroresistant vancomycin-intermediate Staphylococcus aureus and vancomycin-inermediate S.aureus
Cafiso V;BLANDINO, Giovanna;STEFANI, Stefania
2012-01-01
Abstract
We assessed a new screening method, based on -hemolysin production in the presence of 6 mg/liter vancomycin, to distinguishheteroresistant vancomycin-intermediate Staphylococcus aureus (hVISA) and vancomycin-intermediate S. aureus (VISA) fromvancomycin-susceptible S. aureus (VSSA). On 37 clinical methicillin-resistant S. aureus (MRSA) isolates, hVISA and VISA displayedno -hemolysis whereas VSSA displayed strong -hemolysis, showing 91.6% sensitivity. These data, supported by realtimereverse transcription PCR (real-time RT-PCR) highlighting an hld downregulation, i.e., VSSA>hVISA>VISA, define thisnew assay as a valid screening method.File | Dimensione | Formato | |
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