The immunoexpression profile of matrixmetalloproteinase-13 was investigated for thefirst time in dentin of human caries andhealthy teeth. Twelve permanent premolars(10 caries and 2 sound) were decalcified inethylenediaminetetraacetic acid and proces -sed for embedding in paraffin wax. Sections 3-4 μm in thickness were cut and processed forimmunohistochemistry. A mouse monoclonalanti-metalloproteinase-13 antibody was usedfor localisation using an immunoperoxidasetechnique. Dentinal immunoreactivity wasdetected in all teeth; it was weak in soundteeth and strong close to the caries area. Thesein vivo findings suggest a role for metalloproteinase-13 in the development and progressionof adult human dental tissue disorders.
Immunohistochemical analysis of matrix metalloproteinase-13 in human caries dentin
LORETO, CARLA AGATA;MUSUMECI, GIUSEPPE;LEONARDI, Rosalia Maria
2014-01-01
Abstract
The immunoexpression profile of matrixmetalloproteinase-13 was investigated for thefirst time in dentin of human caries andhealthy teeth. Twelve permanent premolars(10 caries and 2 sound) were decalcified inethylenediaminetetraacetic acid and proces -sed for embedding in paraffin wax. Sections 3-4 μm in thickness were cut and processed forimmunohistochemistry. A mouse monoclonalanti-metalloproteinase-13 antibody was usedfor localisation using an immunoperoxidasetechnique. Dentinal immunoreactivity wasdetected in all teeth; it was weak in soundteeth and strong close to the caries area. Thesein vivo findings suggest a role for metalloproteinase-13 in the development and progressionof adult human dental tissue disorders.| File | Dimensione | Formato | |
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