Stereoselective amino acid analysis is still a challenging task. In this work, we report a study on the chiral recognition of D,L-Trp and D,L-His using L-Cys capped silver nanoparticles (AgNPs) and copper(II) ion. The AgNPs have been characterized by TEM, UV-Vis spectra and dynamic light scattering (DLS) measurements and used for chiral discrimination. In the L-Cys capped AgNPs, the alpha-amino and alpha-carboxyl groups of the surface-confined amino acid, besides showing either a negative or a neutral charge as a function of the pH, can coordinate the copper(II) ion, which in turn, binds the L- or D-amino acid present in solution forming diastereoisomeric complexes. The resulting systems have been characterized by UV-Vis spectroscopy, exploiting the zwitterionic nature of the cysteine to obtain enantiodiscrimination by a fine tuning of the pH. The analysis of the UV-Vis data by using a multiwavelength approach allows us to determine the kinetic constants ruling the processes
Fine tuning the pH triggers the enantiorecognition of underivatized amino acids by silver nanoparticles: A novel approach based on the focused use of solution equilibria
CONTINO, Annalinda;MACCARRONE, Giuseppe;ZIMBONE, MASSIMO;MUSUMECI, Paolo;CALCAGNO, Lucia;
2015-01-01
Abstract
Stereoselective amino acid analysis is still a challenging task. In this work, we report a study on the chiral recognition of D,L-Trp and D,L-His using L-Cys capped silver nanoparticles (AgNPs) and copper(II) ion. The AgNPs have been characterized by TEM, UV-Vis spectra and dynamic light scattering (DLS) measurements and used for chiral discrimination. In the L-Cys capped AgNPs, the alpha-amino and alpha-carboxyl groups of the surface-confined amino acid, besides showing either a negative or a neutral charge as a function of the pH, can coordinate the copper(II) ion, which in turn, binds the L- or D-amino acid present in solution forming diastereoisomeric complexes. The resulting systems have been characterized by UV-Vis spectroscopy, exploiting the zwitterionic nature of the cysteine to obtain enantiodiscrimination by a fine tuning of the pH. The analysis of the UV-Vis data by using a multiwavelength approach allows us to determine the kinetic constants ruling the processesFile | Dimensione | Formato | |
---|---|---|---|
Fine tuning the pH triggers the enantiorecognition of underivatized.pdf
solo gestori archivio
Tipologia:
Versione Editoriale (PDF)
Licenza:
Non specificato
Dimensione
1.38 MB
Formato
Adobe PDF
|
1.38 MB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.