The mammalian ovarian follicle is the complex reproductive unit comprising germ cell, somatic cells (Cumulus and Granulosa cells), and follicular fluid: paracrine communication among the different cell types through follicular fluid ensures the development of a mature oocyte ready for fertilization. This paper is focused on non-coding RNAs in ovarian follicles and their predicted role in the pathways involved in oocyte growth and maturation. We determined the expression profiles of microRNAs in human oocytes and follicular fluid by high-throughput analysis and identified 267 microRNAs in follicular fluid and 176 in oocytes. Most of these were follicular fluid microRNAs, while 9 were oocyte specific. By bioinformatic analysis, independently performed on follicular fluid and oocyte microRNAs, we identified the most significant Biological Processes and the pathways regulated by their validated targets. We found many pathways shared between the two compartments and some specific for oocyte microRNAs. Moreover, we found 41 long non-coding RNAs able to interact with oocyte microRNAs and potentially involved in the regulation of folliculogenesis. These data are important in basic reproductive research and could also be useful for clinical applications. In fact, the characterization of non-coding RNAs in ovarian follicles could improve reproductive disease diagnosis, provide biomarkers of oocyte quality in Assisted Reproductive Treatment, and allow the development of therapies for infertility disorders.
|Titolo:||Non-coding RNAs in the Ovarian Follicle|
|Data di pubblicazione:||2017|
|Appare nelle tipologie:||1.1 Articolo in rivista|