Introduction: To asses the antibacterial effectiveness of photon initiated photoacustic streaming (PIPS) of irrigants using an Er:YAG laser in infected root canals. Materials & Methods: One hundred-forty-eight single- rooted extracted teeth were prepared to a size #25.06 taper. After sterilization, all teeth except ten (negative control group) were inoculated with Enterococcus fàecalis. Infected teeth were then randomly divided into 4 test groups (n-32 for each) and 1 positive control group (n-10): pulsed erbium:yAG laser at non-ablative settings for 30 seconds with sterile bi-distilled water or 50% sodium hypochlorite (NaOCl) irrigation (Group A and B respectively); only sterile bi-distilled water or only 5% NaOCI irrigation for 30 seconds (Group C and D respectively); no treated infected teeth (positive control group). Colony-forming units (CFUs) were counted from bacteriologic samples taken before (S1) and after treatment (S2). Results: Group B showed the highest bacteria CFUs reduction that was significantly greater than that of group A and C (P0.05). None of the four groups predictably generated negative sampies, but NaOCI with PIPS activation was superior to the other three groups in this aspect. Conclusions: Er:Yag laser and PIPS technique along with the irrigants resulted highly effective in decontaminating the infected root canals and produced more negative samples than the other groups.

Decontamination efficacy of photon initiated photoacustic streaming (PIPS) of irrigants using low energy laser settings: an in vitro study.

PEDULLA', EUGENIO;Tempera G;Genovese C;Rapisarda E.
2012-01-01

Abstract

Introduction: To asses the antibacterial effectiveness of photon initiated photoacustic streaming (PIPS) of irrigants using an Er:YAG laser in infected root canals. Materials & Methods: One hundred-forty-eight single- rooted extracted teeth were prepared to a size #25.06 taper. After sterilization, all teeth except ten (negative control group) were inoculated with Enterococcus fàecalis. Infected teeth were then randomly divided into 4 test groups (n-32 for each) and 1 positive control group (n-10): pulsed erbium:yAG laser at non-ablative settings for 30 seconds with sterile bi-distilled water or 50% sodium hypochlorite (NaOCl) irrigation (Group A and B respectively); only sterile bi-distilled water or only 5% NaOCI irrigation for 30 seconds (Group C and D respectively); no treated infected teeth (positive control group). Colony-forming units (CFUs) were counted from bacteriologic samples taken before (S1) and after treatment (S2). Results: Group B showed the highest bacteria CFUs reduction that was significantly greater than that of group A and C (P0.05). None of the four groups predictably generated negative sampies, but NaOCI with PIPS activation was superior to the other three groups in this aspect. Conclusions: Er:Yag laser and PIPS technique along with the irrigants resulted highly effective in decontaminating the infected root canals and produced more negative samples than the other groups.
2012
Er:YAG Laser; Enterococcus Faecalis; Decontamination root canals
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/250133
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