The aim of this work was to study 'in vitro' the effects of anticonvulsants Gabapentin (GBP), Carbamazepine (CBZ), Lamotrigine (LTG), Sodium Valproate (VPA), Topiramate (TPM) and Oxcarbazepine (OXC) on nitric oxide (NO) biosynthesis in primary cultures of rat astrocytes. We performed tests to investigate the effects of these drugs at different doses. GBP, CBZ, LTG, VPA, TPM and OXC at concentrations from 1 to 200 μg/ml demonstrated effects on astrocytes, promoting an induction in the biosynthesis of NO, but in any case not as great as Lipopolysaccharides (LPS), used as a control stress-inducing molecule. When the same drugs were added to astrocytes treated with LPS, the biosynthesis of NO increased further only at higher concentrations. These preliminary results may be relevant in elucidating the mechanisms of action, toxicity and/or protective effects of these anticonvulsants.
Nitric oxide release by treated antiepileptic drugs primary rat astrocytes
CARDILE, Venera;RUSSO, Alessandra;PERCIAVALLE, Vincenzo
2000-01-01
Abstract
The aim of this work was to study 'in vitro' the effects of anticonvulsants Gabapentin (GBP), Carbamazepine (CBZ), Lamotrigine (LTG), Sodium Valproate (VPA), Topiramate (TPM) and Oxcarbazepine (OXC) on nitric oxide (NO) biosynthesis in primary cultures of rat astrocytes. We performed tests to investigate the effects of these drugs at different doses. GBP, CBZ, LTG, VPA, TPM and OXC at concentrations from 1 to 200 μg/ml demonstrated effects on astrocytes, promoting an induction in the biosynthesis of NO, but in any case not as great as Lipopolysaccharides (LPS), used as a control stress-inducing molecule. When the same drugs were added to astrocytes treated with LPS, the biosynthesis of NO increased further only at higher concentrations. These preliminary results may be relevant in elucidating the mechanisms of action, toxicity and/or protective effects of these anticonvulsants.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
