The first set of eight polymorphic microsatellites markers was successfully optimized from a partial genomic library enriched for an AC motif of the killifish Aphanius fasciatus Nardo, 1827 (Teleostei, Cyprinodontidae), a brackish-water fish widely distributed along the coasts of the Mediterranean Sea. The developed loci proved to be polymorphic, displayingfrom two to six alleles per locus with expected heterozygosity ranging from 0.277 to 0.752. All loci were under Hardy–Weinberg equilibrium, except Af8 that showed significantheterozygotes deficiency, and there was no evidence of linkage associations betweenany pair of loci. Markers reported here will be potentially useful in monitoring of geneticpopulation structure in this species.

Isolation and characterization of six polymorphic microsatellite markers in Aphanius fasciatus (teleostei, Cyprinodontidae)

FERRITO, Venera;PAPPALARDO, ANNA MARIA
2007-01-01

Abstract

The first set of eight polymorphic microsatellites markers was successfully optimized from a partial genomic library enriched for an AC motif of the killifish Aphanius fasciatus Nardo, 1827 (Teleostei, Cyprinodontidae), a brackish-water fish widely distributed along the coasts of the Mediterranean Sea. The developed loci proved to be polymorphic, displayingfrom two to six alleles per locus with expected heterozygosity ranging from 0.277 to 0.752. All loci were under Hardy–Weinberg equilibrium, except Af8 that showed significantheterozygotes deficiency, and there was no evidence of linkage associations betweenany pair of loci. Markers reported here will be potentially useful in monitoring of geneticpopulation structure in this species.
2007
Aphanius fasciatus; Cyprinodontidae; FIASCO; microsatellites
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/3527
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