The ectopic re-activation of cell cycle in neurons is an early event in the pathogenesis of Alzheimer's disease (AD), which could lead to synaptic failure and ensuing cognitive deficits before frank neuronal death. Cytostatic drugs that act as cyclin-dependent kinase (CDK) inhibitors have been poorly investigated in animal models of AD. In the present study, we examined the effects of flavopiridol, an inhibitor of CDKs currently used as antineoplastic drug, against cell cycle reactivation and memory loss induced by intracerebroventricular injection of A beta(1-42) oligomers in CD1 mice. Cycling neurons, scored as NeuN-positive cells expressing cyclin A, were found both in the frontal cortex and in the hippocampus of A beta-injected mice, paralleling memory deficits. Starting from three days after A beta injection, flavopiridol (0.5, 1 and 3 mg/kg) was intraperitoneally injected daily, for eleven days. Here we show that a treatment with flavopiridol (0.5 and 1 mg/kg) was able to rescue the loss of memory induced by A beta(1-42), and to prevent the occurrence of ectopic cell-cycle events in the mouse frontal cortex and hippocampus. This is the first evidence that a cytostatic drug can prevent cognitive deficits in a non-transgenic animal model of AD. (C) 2016 Elsevier Ltd. All rights reserved.

The antineoplastic drug flavopiridol reverses memory impairment induced by Amyloid-ß1-42 oligomers in mice

Catania, Maria Vincenza;Puzzo, Daniela;Gozzo, Lucia;Palmeri, Agostino;Salomone, Salvatore;Caraci, Filippo;Drago, Filippo
2016

Abstract

The ectopic re-activation of cell cycle in neurons is an early event in the pathogenesis of Alzheimer's disease (AD), which could lead to synaptic failure and ensuing cognitive deficits before frank neuronal death. Cytostatic drugs that act as cyclin-dependent kinase (CDK) inhibitors have been poorly investigated in animal models of AD. In the present study, we examined the effects of flavopiridol, an inhibitor of CDKs currently used as antineoplastic drug, against cell cycle reactivation and memory loss induced by intracerebroventricular injection of A beta(1-42) oligomers in CD1 mice. Cycling neurons, scored as NeuN-positive cells expressing cyclin A, were found both in the frontal cortex and in the hippocampus of A beta-injected mice, paralleling memory deficits. Starting from three days after A beta injection, flavopiridol (0.5, 1 and 3 mg/kg) was intraperitoneally injected daily, for eleven days. Here we show that a treatment with flavopiridol (0.5 and 1 mg/kg) was able to rescue the loss of memory induced by A beta(1-42), and to prevent the occurrence of ectopic cell-cycle events in the mouse frontal cortex and hippocampus. This is the first evidence that a cytostatic drug can prevent cognitive deficits in a non-transgenic animal model of AD. (C) 2016 Elsevier Ltd. All rights reserved.
Alzheimer’s disease
Cell cycle
Flavopiridol
Flavopiridol (PubChem CID: 9910986)
Memory deficit
Oligomers
β-amyloid
Alzheimer Disease
Amyloid beta-Peptides
Animals
Antineoplastic Agents
Cognition Disorders
Cyclin-Dependent Kinases
Disease Models, Animal
Flavonoids
Frontal Lobe
Hippocampus
Male
Memory
Memory Disorders
Mice
Neurons
Peptide Fragments
Piperidines
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/540119
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