Aflatoxins (AFLs), Ochratoxin A (OTA), and Zearalenone (ZEA) are only some of secondary metabolites produced from various filamentous fungi (Aspergillus, Penicillium, and Fusarium), in particular environmental conditions. Micotoxins represent an emergent risk because of toxicity and elevated diffusion that leads to an increment of food contamination. Moreover, they still represent one of the more significant and difficult aspects of food and feeds contamination because qualitative and quantitative valuation about nature of toxic effect has been sufficiently developed only for some effects and for main micotoxins. Whereas still remains much to study about carcinogenic, estrogenic, genotossic, teratogenic, and neurotoxic action. Moreover, to estimate population exposure it is necessary to consider frequencies and contamination levels from micotoxins in the various alimentary matrices and their consumptions. In our study we proposed wheat samples because it is the main ingredient of bread and pasta, basic aliments, and quantitatively substantial of Mediterranean diet.Ten wheat samples, from various areas of East Sicily, was analyzed for micotoxins (AFLs, OTA, ZEA). Analytical methods allow, after extraction with solvents, contemporary purification of AFLs, OTA, and ZEA using immunoaffinity column (IAC); therefore, determination has been carried out by high performance liquid chromatography (HPLC) with fluorescence detector. In particular, AFLs were analyzed, always by fluorescence detector, after postcolumn photochemical derivation.The results show that all samples collected do not contain AFLs whereas OTA and ZEA have been found in the samples to medium concentrations of 0.01 mg/kg and 0.108 mg/kg, respectively.All samples contain OTA and ZEA. So our study confirms the findings of the related studies present in literature. The values found are under of the law limits, but it is not from neglecting the generalized contamination in view of a possible addition of small concentrations present in the single ingredients that compose population diet.
AFLs, OTA and ZEA in wheat samples come from east Sicily
FIORE M;OLIVERI CONTI G;FERRANTE M
2007-01-01
Abstract
Aflatoxins (AFLs), Ochratoxin A (OTA), and Zearalenone (ZEA) are only some of secondary metabolites produced from various filamentous fungi (Aspergillus, Penicillium, and Fusarium), in particular environmental conditions. Micotoxins represent an emergent risk because of toxicity and elevated diffusion that leads to an increment of food contamination. Moreover, they still represent one of the more significant and difficult aspects of food and feeds contamination because qualitative and quantitative valuation about nature of toxic effect has been sufficiently developed only for some effects and for main micotoxins. Whereas still remains much to study about carcinogenic, estrogenic, genotossic, teratogenic, and neurotoxic action. Moreover, to estimate population exposure it is necessary to consider frequencies and contamination levels from micotoxins in the various alimentary matrices and their consumptions. In our study we proposed wheat samples because it is the main ingredient of bread and pasta, basic aliments, and quantitatively substantial of Mediterranean diet.Ten wheat samples, from various areas of East Sicily, was analyzed for micotoxins (AFLs, OTA, ZEA). Analytical methods allow, after extraction with solvents, contemporary purification of AFLs, OTA, and ZEA using immunoaffinity column (IAC); therefore, determination has been carried out by high performance liquid chromatography (HPLC) with fluorescence detector. In particular, AFLs were analyzed, always by fluorescence detector, after postcolumn photochemical derivation.The results show that all samples collected do not contain AFLs whereas OTA and ZEA have been found in the samples to medium concentrations of 0.01 mg/kg and 0.108 mg/kg, respectively.All samples contain OTA and ZEA. So our study confirms the findings of the related studies present in literature. The values found are under of the law limits, but it is not from neglecting the generalized contamination in view of a possible addition of small concentrations present in the single ingredients that compose population diet.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
