Fluoro-edenite (NaCa2Mg5Si7AlO22F2) is a newly discovered fibrous mineral, the presence of which has been correlated with the incidence of mesothelioma that develops among residents in Biancavilla (Catania), a town in eastern Sicily located in the Etna volcanic area. In this study, we used: i) two types of fluoro-edenite, 19 and 27, that have low and high iron content, respectively; ii) tremolite, a member of amphibole asbestos that originates from Calabria (Italy); and iii) crocidolite, that, due to its high toxicity, is one of the most studied asbestos amphiboles. Since pro-inflammatory cytokines are strongly implicated as mediators of asbestos-induced pathophysiological responses, we thought investigate if fluoro-edenite exposure may induce cytokines increase in human alveolar epithelial A549 cells, used as model for studying interaction between environmental particulates/fibers and human respiratory epithelium. Given the role of phosphoinositide-specific phospholipase C (PLC) isozymes in the control of cell growth and differentiation, we were prompted to analyze the expression of some PLC too. The pro-inflammatory cytokine concentrations, such as IL-1β, IL-6 and TNF-α were measured by a specific ELISA kit. The expression of β1 and γ1 PLC was evaluated by Western blot analysis and immunocytochemistry. The results show that the exposure of A549 cells to all experimental fibres ensued IL-1β, IL-6 and TNF-α secretion, overall fluoro-denite 27 and crocidolite. Moreover, they demonstrate an increased expression of both PLC ß1 and γ1 in treated cultures with mineral fibres, even if our data suggest that cytokine release and PLC expression are not strictly linked.
|Titolo:||Increased release of pro-inflammatory cytokines and activation of phosphoinositide-specific phospholipase c in human alveolar epithelial A549 cells treated with mineral fibres|
|Data di pubblicazione:||2009|
|Appare nelle tipologie:||1.5 Abstract in rivista|