2026-09-24T17:31:49Zhttps://www.iris.unict.it/oai/request

oai:www.iris.unict.it:20.500.11769/5831172023-12-15T00:09:27Zcom_20.500.11769_434851com_123456789_40col_20.500.11769_434852

Evidence for enhanced oxidative stress in smokers with severe asthma EMMA, ROSALIA Emma, Rosalia POLOSA, Riccardo MALATINO, Lorenzo Severe asthma, tobacco smoke, oxidative stress, oxidants, antioxidants, FeNO Background As part of the U-BIOPRED project, extensive clinical and biomarker information was collected from cohorts of adult severe asthma smokers/ex-smokers (SAs/ex) and severe asthmatic non-smokers (SAn). Oxidative stress in SAs/ex vs SAn patients was investigated by analysing urinary 8-iso-PGF2a and the mRNA expression of the main pro-oxidant (NOX2; NOSs) and anti-oxidant (SODs; CAT; GPX1) enzymes in the airways, in relation to clinical outcomes. Methods Urine and induced sputum (IS) were obtained from severe asthma patients. A bronchoscopy to obtain bronchial biopsy (BB) and brushing (BBr) was performed in a subset of patients. Urinary 8-iso-PGF2a was quantified using mass spectrometry. IS, BB and BBr were processed for mRNA expression microarray analysis. Results Urinary 8-iso-PGF2a was increased in SAs/ex compared to SAn (p< 0.001) and in current smokers vs ex-smokers and non-smokers (p= 0.004). Sputum mRNA expression of NOX2 were increased in SAs/ex compared to SAn (three probe-sets with p< 0.05). The mRNA expression of antioxidant enzymes was similar between the two severe asthma cohorts in all airway samples. NOS2 mRNA expression was decreased in BBr of SAs/ex compared to SAn (p= 0.029). NOS2 mRNA expression in BBr correlated with FeNO (p< 0.001). FeNO was lower in current-smokers than in ex-smokers (p= 0.007) indicating an effect of active smoking. Conclusions The data suggests evidence of greater systemic oxidative stress in SAs/ex with significant effects on the mRNA expression of NOX2 and NOS2 in the airways of severe asthmatic patients. 2017-11-21 info:eu-repo/semantics/doctoralThesis https://hdl.handle.net/20.500.11769/583117 eng info:eu-repo/semantics/openAccess Università degli studi di Catania place:Catania license:PUBBLICO - Pubblico con Copyright license uri:iris.PUB02