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oai:www.iris.unict.it:20.500.11769/5862182024-01-11T00:28:50Zcom_20.500.11769_434851com_123456789_40col_20.500.11769_434852

Diet, genetic and epigenetic signatures in women of childbearing age from a Mediterranean population: perspectives for public health QUATTROCCHI, ANNALISA Quattrocchi, Annalisa AGODI, ANTONELLA PAOLA CONDORELLI, Daniele Filippo Global DNA methylation folate deficiency mediterranen diet The aim of the study was to determine the association between Mediterranean diet (MD), folate intake and global DNA methylation in women of childbearing age. A total of 177 women were enrolled in a cross-sectional study. Folate intake and MD were assessed by a validated food frequency questionnaire. LINE-1 methylation level in leukocytes was measured by pyrosequencing. Women reported poor adherence to MD (mean value 4.1) and high prevalence of folate deficiency (83.1%). Mean level of LINE-1 methylation was 65.3%. A strong association between folate deficiency and global hypomethylation, was found: women with folate deficiency had a 4 fold increased risk to be hypometylated compared with women with no folate deficiency (OR = 4; 95% CI: 1.3 11.8). In multivariate analysis, the association decreased with a borderline confidence interval (p=0.053, OR = 3; 95% CI: 1 3.3). Poor adherence to MD was not directly associated with global methylation level, however, women whose consumption of fruit was below the median (201 gr/day) had a 2.8 fold increased risk to be hypometylated in comparison with those women whose consumption was above the median value (OR = 2.8; 95% CI: 1.3 5.8; p=0.019). In multivariate logistic regression analysis, results, adjusted for age, nutritional status, smoking habits and dietary caloric intake, showed a significant relationship between low fruits intake and low methylation level (p = 0.006; OR = 3.8; 95% CI: 1.5 9.7). The inverse association between high consumption of fruit, which represents a mainsource of folate, and DNA hypomethylation, suggests that dietary pattern characterized by a high intake of fruits and, in a broader view, great adherence to MD may protect against DNA hypomethylation. 2013-12-10 info:eu-repo/semantics/doctoralThesis https://hdl.handle.net/20.500.11769/586218 eng info:eu-repo/semantics/openAccess Università degli studi di Catania place:Catania license:PUBBLICO - Pubblico con Copyright license uri:iris.PUB02