To main pathways of the apoptosis process have been identified: the extrinsic or (death receptor- mediated) and the intrinsic (or mitochondrial) pathway. While apoptotic cells death, through extrinsic pathway, has just been demonstrated in degenerated temporomandibular discs, no literature data has been yet published on the involvement of the intrinsic pathway in these samples. Thus the present study was conducted in order to investigate whether the intrinsic pathway participates in the activation of the apoptotic process in patients with temporomandibular joint internal derangement and anterior disc displacement without reduction (TMJ ID with ADDwoR). The apoptosis activation process was studied in 15 patients with internal derangement and 6 unaffected discs by means of Bcl-2–associated X protein (bax), B-cell lymphoma 2 (bcl-2), cytochrome c and caspase 9 immunoexpression; a correlation between the immunohistochemical findings and the degree of disc damage was also conduced. The results showed a bax, cytochrome c and caspase 9 strong cellular immunoexpression accompanied by a negative bcl-2 immunostaining in affected discs. The degrees of bax, cytochrome c and caspase 9 immunostaining were significant (p<0.01) and correlated with HDS data (Spearman’s rho=0.617). None of the temporomandibular joint discs sections with internal derangement were immonopositive for bcl-2. The outcomes of the present study suggest that in human TMJ ID with ADDwoR, cells apoptosis occurs, at least partly, via the mitochondrial pathway which contribute to the developing subsequent disc degeneration. These data may have clinical implications and help improvement treatment strategies.
|Titolo:||Apoptosis of temporomandibular joint disc with internal derangement involves mitochondrial-dependent pathways. An in vivo study|
|Autori interni:||MUSUMECI, GIUSEPPE|
LORETO, CARLA AGATA
|Data di pubblicazione:||2013|
|Rivista:||ACTA ODONTOLOGICA SCANDINAVICA|
|Appare nelle tipologie:||1.1 Articolo in rivista|