Transgenic approach represents a valid and robust tool for genetic improvement of important traits, as yield and quality, in wheat. Recently Sestili and colleagues [1] have produced high amylose transgenic lines by silencing SBEIIa genes in two cultivars of durum wheat (Svevo and Ofanto) using two different methods of transformation (particle bombardment for cv Svevo and Agrobacterium for cv Ofanto). In the present work, unintended effects, due to gene silencing, have been investigated in the metabolic fraction extracted from immature (15 DPA) and mature (36 DPA) grain of the two transformed cultivars and corresponding normal counterparts. Several differentially expressed spots have been identified and analyzed by mass spectrometry. These spots can be grouped in three large classes, including carbohydrates metabolism, defense mechanisms and biosynthetic pathways. In conclusion the two methods of genetic transformation had similar secondary effects on metabolic protein fraction of the two transgenic lines. Although the comparative analysis transgenic vs wild type has highlighted some differences, these were similar to those observed in comparative analysis of conventional crops grown in different years and environmental conditions [2-3]. References: [1] Sestili F, Janni M, Doherty A, Botticella E, D’Ovidio R, Masci S, Jones H, Lafiandra D. Increasing the amylose content of durum wheat through silencing of the SBEIIa genes. BMC Plant Biology. 2010;10:144. [2] Barros E, Lezar S, Anttonen MJ, Van Dijk JP, Rohlig RM, Kok EJ, Engel KH. Comparison of two GM maize varieties with a near-isogenic non-GM variety using transcriptomics, proteomics and metabolomics. Plant Biotechnol Journal. 2010;8(4):436-51. [3] Coll A, Nadal A, Collado R, Capellades G, Kubista M, Messeguer J, Pla M. Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices. Plant Mol Biology. 2010;73:349–362
Comparative proteomic analysis of metabolic proteins of two durum wheat cultivars and their derived transgenic lines
SALETTI, Rosaria;CUNSOLO, VINCENZO;MUCCILLI, VERA;FOTI, Salvatore;
2012-01-01
Abstract
Transgenic approach represents a valid and robust tool for genetic improvement of important traits, as yield and quality, in wheat. Recently Sestili and colleagues [1] have produced high amylose transgenic lines by silencing SBEIIa genes in two cultivars of durum wheat (Svevo and Ofanto) using two different methods of transformation (particle bombardment for cv Svevo and Agrobacterium for cv Ofanto). In the present work, unintended effects, due to gene silencing, have been investigated in the metabolic fraction extracted from immature (15 DPA) and mature (36 DPA) grain of the two transformed cultivars and corresponding normal counterparts. Several differentially expressed spots have been identified and analyzed by mass spectrometry. These spots can be grouped in three large classes, including carbohydrates metabolism, defense mechanisms and biosynthetic pathways. In conclusion the two methods of genetic transformation had similar secondary effects on metabolic protein fraction of the two transgenic lines. Although the comparative analysis transgenic vs wild type has highlighted some differences, these were similar to those observed in comparative analysis of conventional crops grown in different years and environmental conditions [2-3]. References: [1] Sestili F, Janni M, Doherty A, Botticella E, D’Ovidio R, Masci S, Jones H, Lafiandra D. Increasing the amylose content of durum wheat through silencing of the SBEIIa genes. BMC Plant Biology. 2010;10:144. [2] Barros E, Lezar S, Anttonen MJ, Van Dijk JP, Rohlig RM, Kok EJ, Engel KH. Comparison of two GM maize varieties with a near-isogenic non-GM variety using transcriptomics, proteomics and metabolomics. Plant Biotechnol Journal. 2010;8(4):436-51. [3] Coll A, Nadal A, Collado R, Capellades G, Kubista M, Messeguer J, Pla M. Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices. Plant Mol Biology. 2010;73:349–362I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
