The current work describes a hydrogel route for the amine functionalization of polycaprolactone scaffolds with a honeycomb structure (PCLhc). The functionalization takes place after contact with fresh liquid hydrogels. After gelification, hydrogels are removed for evaluation of the transferred active molecules. The formation of hybrids with variable agarose/aminopropyltriethoxysilane (APTS) ratio allowing gradual grafting was evaluated initially onto Si planar surfaces. Analysis of the line shape of Si 2p XPS allowed confirming on the one hand that Si-O(x) contribution related to APTS increases as Si-Si signal due to Si (100) substrate decreases for increasing aminosilane load in the gel. On the other hand, the line shape of C 1s and N 1s core level spectra were characteristic of those related to APTS functionalization, with a particular label due to partial agarose transfer to the substrate. In this stage, PCLhc scaffolds were activated with the agarose-APTS hybrids. A peeling after gelification gave rise not only to amine-activated PCLhc structures, but also to hc structure replicas on the contacting surfaces of the agarose-APTS hydrogels. In order to evaluate the activity of modified PCLhc scaffolds, a labeled mouse antibody was immobilized and its functionalization evaluated by fluorescence microscopy. Copyright (C) 2010 John Wiley & Sons, Ltd.
Application of hybrid agarose-aminosilane gels to the biofunctionalization of honeycomb-structured polycaprolactone scaffolds
|Titolo:||Application of hybrid agarose-aminosilane gels to the biofunctionalization of honeycomb-structured polycaprolactone scaffolds|
|Data di pubblicazione:||2010|
|Appare nelle tipologie:||1.1 Articolo in rivista|