Background. Keeping platelet metabolism and function at high qualitative level during storage is mandatory to ensure clinical effectiveness of platelet transfusion. To this purpose, new storage conditions and additives are constantly evaluated. Using glucose as a substrate is controversial because of its potential connection with increase in lactate production and decrease in pH, both parameters triggering platelet lesion during storage. Materials and Methods. In this study, we analyzed the morphological status and metabolic profile after 0, 2, 4, 6 and 8 days of platelet stored in autologous plasma enriched with increasing glucose concentrations (13.75, 27.5 and 55 mM). At each time point, high energy phosphates (ATP, GTP, ADP, AMP), oxypurines (hypoxanthine, xanthine, uric acid), lactate, pH, mitochondrial function, cell lysis and morphology, were evaluated. Results. Data showed a significant dose-dependent improvement of the different parameters in platelets stored with increasing glucose, compared to what detected in controls. Interestingly, this phenomenon was more remarkable at the highest level of glucose tested and in the period of time generally used for platelet transfusion (0-6 days). Conclusion. These results indicate that glucose addition during platelet storage ameliorates in a dose-dependent manner the biochemical parameters related to energy metabolism and mitochondrial function. Since in our experiments there was no correspondence between glucose addition, lactate increase and pH decrease, it is conceivable that platelet derangement during storage is not directly caused by glucose through an increase of anaerobic glycolysis, but rather to a loss of mitochondrial functions caused by reduced substrate availability.
|Titolo:||Glucose ameliorates the metabolic profile and mitochondrial function of platelet concentrates during storage in autologous plasma|
|Data di pubblicazione:||2013|
|Appare nelle tipologie:||1.1 Articolo in rivista|