AbstractBackground and Aims Vitamin D (VitD) is emerging for putative immunomodulatory and antifibrotic effect, and its deficiency is associated with NAFLD and sarcopenia. Olive oil is deemed as a factor of the effect of the Mediterranean diet nutritional profile. We investigated the role of VitD supplementation or deprivation in sedentary rats fed standard diet, high fat diet with animal fat or extra-virgin olive oil (EVOO), studying the development of fatty liver disease and muscle tissue change.Methods In this study 28 male rats, 7–9 weeks of age, 200 g of body weight, on a 12-hour light/dark cycle and ad libitum access to assigned diet and water, were randomly divided in 7 groups and fed for 10 weeks with 7 different dietary profiles [Regular diet (1); Regular diet + VitD (2), Regular diet VitD deprived (3); High fat/butter + VitD (4) and VitD deprived (5); High fat/EVOO plus VItD (6) and VitD deprived (7)]. Microscopic evaluation and histochemical study of liver and muscle was performed. The NAFLD Activity Score (NAS), ranging from 0 to 8, i.e. for steatosis (0–3), lobular inflammation (0–3), and ballooning (0–2), and the automated muscle fibers image analysis were used.Results No difference was found among the 7 groups for final body weight gain, although rats fed a standard diet VitD deprived (3) had the final lower weight; the high fat/EVOO plus VitD rats (5) were heavier vs. controls (p 0,07). Rats on high fat/butter diet, both VitD enriched (4) or deprived (5), and high fat/EVOO VitD deprived (7) developed at last significantly greater NAS. Differently, rats on regular diet (1), notwithstanding with the adjunct (2) or deprivation of VitD (3), and rats fed with high fat/EVOO VitD enriched diet (6) did not show evidence of NAS increase. This last group (high fat/EVOO plus VitD) showed slight lobular inflammation without signs of steatosis, and their muscle fibers were hypertrophic respect to control and to other groups. In contrast, in group 5 (high fat/butter VitD deprived) the fibers were hypotrophic.Conclusions VitD seems to be protective against steatosis in rats fed with EVOO-based high fat diet, but not with butter-based high fat diet. Moreover, VitD deficiency is associated with muscle fibers hypotrophy, effect counteracted by olive oil-based diet. Extra-virgin olive oil and Vitamin D may share a protective role on liver and muscle in sedentary rats against the induction of fatty liver and sarcopenia.
|Titolo:||Preliminary data on the effects of high fat diet, olive oil and vitamin D on fatty liver and muscle in an experimental model|
MUSUMECI, GIUSEPPE (Corresponding)
|Data di pubblicazione:||2017|
|Appare nelle tipologie:||1.5 Abstract in rivista|