The gut microbiome is not a silent and isolated ecosystem but exerts several physiological and immunological functions into a complex ‘super-organism’ with which has co-evolved. The lactobacilli, protagonists of this ecosystem, have been used as an effective therapy for treatment of several pathological conditions displaying an overall positive safety profile. The lactobacilli administration has shown to induce qualitative and quantitative modifications in the human gastrointestinal microbial ecosystem with encouraging perspectives in counteracting pathologys. Despite great attention, the understanding of the biological processes involved in the beneficial effects of lactobacilli strains is still limited. To this end, the present study investigated the growth-inhibitory effects of Lactobacillus rhamnosus GG against tumour cell lines. Administration of cell-free supernatant from Lactobacillus rhamnosus GG on human (HT29, HCT-116, Caco-2) colon carcinoma cell lines and human (A375) melanoma cells raised a significant concentration-dependent anti-proliferative effect, determined by cell viability assays. Specifically, cell-free supernatant from Lactobacillus rhamnosus GG did not induce apoptotic cell death in tumour cell lines as revealed by annexin V and propidium iodide staining but rather induced G2/M cell cycle arrest. In addition, CFS-LGG administration combined with chemotherapy drugs has potentiated their action. Taken together, these findings provide evidence for beneficial anti-proliferative and tumour inhibitory effects derivated by Lactobacillus rhamnosus GG.
Il microbioma intestinale non è un ecosistema silenzioso e isolato ma esercita diverse funzioni fisiologiche e immunologiche in un complesso "superorganismo" con il quale si è coevoluto. I lattobacilli, protagonisti di questo ecosistema, sono stati utilizzati come terapia efficace per il trattamento di diverse condizioni patologiche poichè mostrano un profilo di sicurezza complessivamente positivo. La somministrazione di lattobacilli ha dimostrato di indurre modifiche qualitative e quantitative nell'ecosistema microbico gastrointestinale umano con prospettive incoraggianti nel contrastare le patologie. Nonostante la grande attenzione, la comprensione dei processi biologici coinvolti negli effetti benefici dei ceppi di lattobacilli è ancora limitata. A tal fine, il presente studio ha investigato gli effetti inibitori di Lactobacillus rhamnosus GG sulla proliferazine di linee cellulari tumorali. Il trattamento di linee cellulari di carcinoma del colon umano (HT29, HCT-116, Caco-2) e cellule di melanoma umano (A375)con surnatante di Lactobacillus rhamnosus GG ha mostrato un significativo effetto antiproliferativo dipendente dalla concentrazione, determinato con saggi di vitalità cellulare . In particolare, il surnatante di Lactobacillus rhamnosus GG non ha indotto la morte cellulare per apoptosi nelle linee cellulari tumorali come rivelato mediante annessina V e colorazione con ioduro di propidio, ma piuttosto ha indotto l'arresto del ciclo cellulare in fase G2 / M. Inoltre, la somministrazione di CFS-LGG combinata con farmaci chemioterapici ha potenziato la loro azione. Questi risultati forniscono la prova di effetti benefici del Lactobacillus rhamnosus GG con attività antiproliferativa e inibitoria delle cellule tumorali.
Effetti antiproliferativi dei surnatanti di Lactobacillus Rhamnosus GG su cellule tumorali / Salemi, Rossella. - (2020 Dec 18).
Effetti antiproliferativi dei surnatanti di Lactobacillus Rhamnosus GG su cellule tumorali
SALEMI, ROSSELLA
2020-12-18
Abstract
The gut microbiome is not a silent and isolated ecosystem but exerts several physiological and immunological functions into a complex ‘super-organism’ with which has co-evolved. The lactobacilli, protagonists of this ecosystem, have been used as an effective therapy for treatment of several pathological conditions displaying an overall positive safety profile. The lactobacilli administration has shown to induce qualitative and quantitative modifications in the human gastrointestinal microbial ecosystem with encouraging perspectives in counteracting pathologys. Despite great attention, the understanding of the biological processes involved in the beneficial effects of lactobacilli strains is still limited. To this end, the present study investigated the growth-inhibitory effects of Lactobacillus rhamnosus GG against tumour cell lines. Administration of cell-free supernatant from Lactobacillus rhamnosus GG on human (HT29, HCT-116, Caco-2) colon carcinoma cell lines and human (A375) melanoma cells raised a significant concentration-dependent anti-proliferative effect, determined by cell viability assays. Specifically, cell-free supernatant from Lactobacillus rhamnosus GG did not induce apoptotic cell death in tumour cell lines as revealed by annexin V and propidium iodide staining but rather induced G2/M cell cycle arrest. In addition, CFS-LGG administration combined with chemotherapy drugs has potentiated their action. Taken together, these findings provide evidence for beneficial anti-proliferative and tumour inhibitory effects derivated by Lactobacillus rhamnosus GG.File | Dimensione | Formato | |
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