In recent years, Insulin-Degrading Enzyme (IDE) has drawn attention because of its capability to degrade β-amyloid peptides and it is currently studied as a pharmacological target for Alzheimer's disease and type II diabetes. Our group has already reported that IDE activity towards insulin and Aβ can be modulated by metal ions such as copper(I), copper(II) and zinc.1-3 In this work, we have investigated how copper and zinc modulate the activity of IDE towards other substrates such as amylin and bradykinin with a new perspective. Attention is devoted to the cryptic peptides generated by IDE from the different substrates and results show that metal ions have a large impact not only on the overall enzyme activity but also on its substrates cryptomes. References 1. G. Grasso, A. Pietropaolo, G. Spoto, G. Pappalardo, G. R. Tundo, C. Ciaccio, M. Coletta, E. Rizzarelli, Chem.- Eur. J. 17 (2011) 2752-2762. 2. G. Grasso, F. Salomone, G. R. Tundo, G. Pappalardo, C. Ciaccio, G. Spoto, A. Pietropaolo, M. Coletta, E. Rizzarelli, J. Inorg. Biochem. 117 (2012) 351-358. 3. F. Bellia, A. Pietropaolo, G. Grasso, J. Mass Spectrom. 48 (2013) 135-140.
Metal ions modulate the cryptic peptides generated by insulin-degrading enzyme in a substrate-dependent manner
GRASSO, GIUSEPPE;
2015-01-01
Abstract
In recent years, Insulin-Degrading Enzyme (IDE) has drawn attention because of its capability to degrade β-amyloid peptides and it is currently studied as a pharmacological target for Alzheimer's disease and type II diabetes. Our group has already reported that IDE activity towards insulin and Aβ can be modulated by metal ions such as copper(I), copper(II) and zinc.1-3 In this work, we have investigated how copper and zinc modulate the activity of IDE towards other substrates such as amylin and bradykinin with a new perspective. Attention is devoted to the cryptic peptides generated by IDE from the different substrates and results show that metal ions have a large impact not only on the overall enzyme activity but also on its substrates cryptomes. References 1. G. Grasso, A. Pietropaolo, G. Spoto, G. Pappalardo, G. R. Tundo, C. Ciaccio, M. Coletta, E. Rizzarelli, Chem.- Eur. J. 17 (2011) 2752-2762. 2. G. Grasso, F. Salomone, G. R. Tundo, G. Pappalardo, C. Ciaccio, G. Spoto, A. Pietropaolo, M. Coletta, E. Rizzarelli, J. Inorg. Biochem. 117 (2012) 351-358. 3. F. Bellia, A. Pietropaolo, G. Grasso, J. Mass Spectrom. 48 (2013) 135-140.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
