Current developments in the field of bioanalytical tools for research and diagnostic purposes tend to propose innovative and multifunctional solutions for studies of biomolecular interactions. Main requirements are expressed not only by sensitive and multiplex detection of binding phenomena. Also reliable identification of interactants is of big interest and opens up to many possible applications. In this scope, integration of surface plasmon resonance (SPR) assays with mass spectrometry methods provides high potential of analytical power. In this work we present development of silica/gold nanostructured layers as a versatile platform enabling analysis by both SPR and MS in imaging mode. Nanostructured gold substrates were prepared upon colloidal lithography and plasma-assisted processes whereas nanometric silica coating was obtained through sol-gel protocol from tetraethyl orthosilicate (TEOS) as precursor. Such surfaces were furthermore modified by devoted chemistries in order to allow addressing of biomolecules. We have therefore reported on analysis of interaction between immobilized model peptides and related target proteins by SPR imaging technique. Eventually silica films were modified by organic matrix molecules and provided detection of model analytes by MS imaging approach which has been demonstrated in this study. © 2015, Avestia Publishing. All rights reserved.
Nanostructured gold surfaces coated by silica-based layers as SPR and MS imaging platform
Spampinato, V.;
2015-01-01
Abstract
Current developments in the field of bioanalytical tools for research and diagnostic purposes tend to propose innovative and multifunctional solutions for studies of biomolecular interactions. Main requirements are expressed not only by sensitive and multiplex detection of binding phenomena. Also reliable identification of interactants is of big interest and opens up to many possible applications. In this scope, integration of surface plasmon resonance (SPR) assays with mass spectrometry methods provides high potential of analytical power. In this work we present development of silica/gold nanostructured layers as a versatile platform enabling analysis by both SPR and MS in imaging mode. Nanostructured gold substrates were prepared upon colloidal lithography and plasma-assisted processes whereas nanometric silica coating was obtained through sol-gel protocol from tetraethyl orthosilicate (TEOS) as precursor. Such surfaces were furthermore modified by devoted chemistries in order to allow addressing of biomolecules. We have therefore reported on analysis of interaction between immobilized model peptides and related target proteins by SPR imaging technique. Eventually silica films were modified by organic matrix molecules and provided detection of model analytes by MS imaging approach which has been demonstrated in this study. © 2015, Avestia Publishing. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
