The approach of non-covalent conjugation has recently been used and a number of metal complexes have been encaged in a protein scaffold to form functional metallo proteins. Here we investigate a novel artificial superoxide dismutase enzyme based on the non-covalent conjugation of bovine serum albumin and the manganese(III) complex of N,N'-bis(salicylidene)-3,4-diaminobenzoic acid. We report the intriguing ability of the albumin environment to increase the SOD activity of the inorganic complex. We also determined the binding constant with BSA by CD spectroscopy. (C) 2011 Elsevier Masson SAS. All rights reserved.
|Titolo:||A novel artificial superoxide dismutase: Non-covalent conjugation of albumin with a Mn(III) salophen type complex|
|Autori interni:||VECCHIO, Graziella|
|Data di pubblicazione:||2011|
|Rivista:||EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY|
|Appare nelle tipologie:||1.1 Articolo in rivista|