Background. TRAIL is a trans membrane protein that induces apoptosis in various tissues including alveolar bone. TRAIL expression could be induced by several methods like RANKL administration and scraping technique. Accordingly we evaluated TRAIL and its receptors DR5 and DcR2 expression in osteoclast like cells to verify TRAIL induced effects on osteoclast life span in order to assess its relationship to orthodontic tooth movement.Materials and Methods. Osteoclast-like cells were differentiated from cells of a mouse hematopoietic cell line, by stimulation with recombinant RANKL. Cells were stimulated with 70 ng/ml GST-RANKL and scraping for 24 hrs (time T1) 72 hrs (time T2) and 5 days (time T3) then immunostaining for TRAIL, death receptors DR5 and DcR2 were performed on control and treated cells.Results. Osteoclast-like cells treated with RANKL 70 ng/ml and scraping method showed a significant increase of TRAIL expression both with the immunocytochemical analysis and Western blotting at 24 hrs (T1) and 72 hrs (T3). Furthermore, the peaks of expression of TRAIL at T1 and T3 corresponded respectively to the peak of its receptors DcR2 and DR5. Conclusions. Our findings might have clinical relevance in orthodontics because a better understanding of the mechanism that regulates tooth movement represents a fundamental step to improve orthodontic technique.

Expression of TRAIL and its receptors DR5 and DcR2 in orthodontic tooth movement

CARDILE, Venera
Primo
;
MUSUMECI, GIUSEPPE
Secondo
;
LEONARDI, Rosalia Maria;LORETO, CARLA AGATA
Ultimo
2013

Abstract

Background. TRAIL is a trans membrane protein that induces apoptosis in various tissues including alveolar bone. TRAIL expression could be induced by several methods like RANKL administration and scraping technique. Accordingly we evaluated TRAIL and its receptors DR5 and DcR2 expression in osteoclast like cells to verify TRAIL induced effects on osteoclast life span in order to assess its relationship to orthodontic tooth movement.Materials and Methods. Osteoclast-like cells were differentiated from cells of a mouse hematopoietic cell line, by stimulation with recombinant RANKL. Cells were stimulated with 70 ng/ml GST-RANKL and scraping for 24 hrs (time T1) 72 hrs (time T2) and 5 days (time T3) then immunostaining for TRAIL, death receptors DR5 and DcR2 were performed on control and treated cells.Results. Osteoclast-like cells treated with RANKL 70 ng/ml and scraping method showed a significant increase of TRAIL expression both with the immunocytochemical analysis and Western blotting at 24 hrs (T1) and 72 hrs (T3). Furthermore, the peaks of expression of TRAIL at T1 and T3 corresponded respectively to the peak of its receptors DcR2 and DR5. Conclusions. Our findings might have clinical relevance in orthodontics because a better understanding of the mechanism that regulates tooth movement represents a fundamental step to improve orthodontic technique.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11769/10483
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