The expression and functional properties of connexin36 (Cx36) were examined in two communication-deficient cell lines (N2A-neuroblastoma and PC- 12 cells) transfected with Cx36 and in hippocampal neurons that express the connexin endogenously. Transfected cells expressed the expected 2.9 kb Cx36 transcript and Cx36 immunoreactivity, whereas nontransfected cells were devoid of Cx36. The relationship between steady-state junctional conductance (g(j)) and transjunctional voltage was well described by a two-state Boltzmann equation. The half-inactivation voltage (V0), the ratio of minimal to maximal g(j) (g(min)/g(max)), and the equivalent gating charge were ± 75 mV, 0.55, and 1.75, respectively, indicating that Cx36 exhibits very low voltage sensitivity. Conductance of single Cx36 channels measured with patch pipettes containing 130 mM CsCl was 10-15 pS (n = 15 cell pairs); despite this low unitary conductance, Cx36 channels were permeable to the dye Lucifer yellow. Hippocampal neurons expressed Cx36 both in vivo and in culture. The electrophysiological properties of channels in cultured hippocampal neurons were similar to those of the channels expressed by the transfected cell lines, and the neuronal channels were similarly permeable to Lucifer yellow. The unique combination of weak voltage sensitivity, small unitary conductance, and permeation by anions as large as second messenger molecules endows Cx36 gap junction channels with properties well suited for mediating flexible electrical and biochemical interactions between neurons.
|Titolo:||Functional Properties of Channels Formed by the Neuronal Gap Junction Protein Connexin36|
|Data di pubblicazione:||1999|
|Citazione:||Functional Properties of Channels Formed by the Neuronal Gap Junction Protein Connexin36 / M. SRINIVAS; R. ROZENTAL; T. KOJIMA; R. DERMIETZEL; M. MEHLER; CONDORELLI D; J. A. KESSLER; D. C. SPRAY. - In: THE JOURNAL OF NEUROSCIENCE. - ISSN 0270-6474. - 19:22(1999), pp. 9848-9855.|
|Appare nelle tipologie:||1.1 Articolo in rivista|