EGF family growth factors consists of growth factors, such as transforming growth factor (TGF)-a, heparinbindingEGF-like growth factor (HB-EGF), amphiregulin (AR) and epiregulin, autocrine growth factors in normal human keratinocytes.HB-EGF is mitogen for epithelial cells and like other members of the EGF family, HB-EGF exerts its biological effects via interactionwith the EGF receptor (EGFR). HB-EGF is an autocrine growth factor for human keratinocytes, and has a possible role as a paracrinegrowth factor for fibroblast. Our report concerning immunohistochemical localization of HB-EGF in normal skin by using the streptavidinperoxidase(HRP) conjugate method, confirms previous data, revealing specific patterns of HB-EGF localization. Identification of HBEGFin cells of epithelial origin suggests its autocrine and/or paracrine roles in epithelial cell maintenance. Our report especially wantsto give a technical contribution, easy to manage and with evident results. A simple technique that does not require use of sophisticatedequipment.
|Titolo:||Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate Method|
|Data di pubblicazione:||2011|
|Citazione:||Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate Method / Castrogiovanni, Paola; Mazzone, Venera; Imbesi, R.. - In: INTERNATIONAL JOURNAL OF MORPHOLOGY. - ISSN 0717-9502. - 29 (4):(Fasc. 4)(2011), pp. 1162-1167.|
|Appare nelle tipologie:||1.1 Articolo in rivista|