EGF family growth factors consists of growth factors, such as transforming growth factor (TGF)-a, heparinbindingEGF-like growth factor (HB-EGF), amphiregulin (AR) and epiregulin, autocrine growth factors in normal human keratinocytes.HB-EGF is mitogen for epithelial cells and like other members of the EGF family, HB-EGF exerts its biological effects via interactionwith the EGF receptor (EGFR). HB-EGF is an autocrine growth factor for human keratinocytes, and has a possible role as a paracrinegrowth factor for fibroblast. Our report concerning immunohistochemical localization of HB-EGF in normal skin by using the streptavidinperoxidase(HRP) conjugate method, confirms previous data, revealing specific patterns of HB-EGF localization. Identification of HBEGFin cells of epithelial origin suggests its autocrine and/or paracrine roles in epithelial cell maintenance. Our report especially wantsto give a technical contribution, easy to manage and with evident results. A simple technique that does not require use of sophisticatedequipment.