EGF family growth factors consists of growth factors, such as transforming growth factor (TGF)-a, heparinbindingEGF-like growth factor (HB-EGF), amphiregulin (AR) and epiregulin, autocrine growth factors in normal human keratinocytes.HB-EGF is mitogen for epithelial cells and like other members of the EGF family, HB-EGF exerts its biological effects via interactionwith the EGF receptor (EGFR). HB-EGF is an autocrine growth factor for human keratinocytes, and has a possible role as a paracrinegrowth factor for fibroblast. Our report concerning immunohistochemical localization of HB-EGF in normal skin by using the streptavidinperoxidase(HRP) conjugate method, confirms previous data, revealing specific patterns of HB-EGF localization. Identification of HBEGFin cells of epithelial origin suggests its autocrine and/or paracrine roles in epithelial cell maintenance. Our report especially wantsto give a technical contribution, easy to manage and with evident results. A simple technique that does not require use of sophisticatedequipment.

Immunolocalization of HB-EGF in Human Skin by Streptavidin-Peroxidase (HRP) Conjugate Method

CASTROGIOVANNI, Paola
Primo
;
MAZZONE, Venera;Imbesi R.
Ultimo
2011-01-01

Abstract

EGF family growth factors consists of growth factors, such as transforming growth factor (TGF)-a, heparinbindingEGF-like growth factor (HB-EGF), amphiregulin (AR) and epiregulin, autocrine growth factors in normal human keratinocytes.HB-EGF is mitogen for epithelial cells and like other members of the EGF family, HB-EGF exerts its biological effects via interactionwith the EGF receptor (EGFR). HB-EGF is an autocrine growth factor for human keratinocytes, and has a possible role as a paracrinegrowth factor for fibroblast. Our report concerning immunohistochemical localization of HB-EGF in normal skin by using the streptavidinperoxidase(HRP) conjugate method, confirms previous data, revealing specific patterns of HB-EGF localization. Identification of HBEGFin cells of epithelial origin suggests its autocrine and/or paracrine roles in epithelial cell maintenance. Our report especially wantsto give a technical contribution, easy to manage and with evident results. A simple technique that does not require use of sophisticatedequipment.
Growth factors; Keratinocytes; Streptavidin-peroxidase conjugate
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/11548
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