The reaction products of five peptides with diethylpyrocarbonate, a site-specific reagent for histidine residues and amino groups, have been synthesized, isolated by high-performance liquid chromatography (HPLC) and identified by fast-atom bombardment (FAB) mass spectrometry. Peptides containing histidine, as well as compounds resulting from the N-carboethoxylation of terminal amino groups and the histidine imidazole ring, provide a derivative produced by cleavage of the histidine heterocyclic ring through a Bamberger reaction. The main mass spectral features of these derivatives are discussed in comparison with structurally related compounds. HPLC along with FAB mass spectrometry shows itself to be a suitable technique for the characterization of these structures.
|Titolo:||FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY OF PEPTIDE DERIVATIVES WITH DIETHYLPYROCARBONATE|
|Autori interni:||SALETTI, Rosaria|
|Data di pubblicazione:||1991|
|Rivista:||RAPID COMMUNICATIONS IN MASS SPECTROMETRY|
|Appare nelle tipologie:||1.1 Articolo in rivista|