The paper reports a spectrophotometric methodology to determine the anthocyanin content in blood orange juices. The criterion of difference of absorbances at 510 nm in two aqueous solutions (pH 1 and pH 4.5) revealed the real concentration of free anthocyanins, using an absorption coefficient obtained from the purest cyanidin-3-glucoside standard. The quantity of pigments is expressed as concentration of cyanidin-3-glucoside, the most important anthyanin in blood orange juices. The method was applied to fresh and concentrated processed juices and was controlled by HPLC anthocyanin analysis in the same juices.
A simple and reliable method for determining anthocyanins in blood orange juices
FALLICO, Biagio;
1994-01-01
Abstract
The paper reports a spectrophotometric methodology to determine the anthocyanin content in blood orange juices. The criterion of difference of absorbances at 510 nm in two aqueous solutions (pH 1 and pH 4.5) revealed the real concentration of free anthocyanins, using an absorption coefficient obtained from the purest cyanidin-3-glucoside standard. The quantity of pigments is expressed as concentration of cyanidin-3-glucoside, the most important anthyanin in blood orange juices. The method was applied to fresh and concentrated processed juices and was controlled by HPLC anthocyanin analysis in the same juices.File in questo prodotto:
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Rapisarda et al. Agrochimica 1994.pdf
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