Inhibitors of Poly(ADP-ribose) polymerases (PARPs) have been proposed as promising chemotherapeutic agents in inflammatory, degenerative, and neoplastic diseases. To further substantiate this proposal, we tested the biomolecular effects of PARP-1 inhibitors DPQ and PJ34 on human glioblastoma (GBM) cells, induced to a proinflammatory state with Lipopolysaccharide (LPS) and Interferon-γ (INF-γ). PARP-1 expression was evaluated by laser scanning confocal microscopy (LSM); nitrite production, LDH release, cell viability were also determined. LSM of A172, SNB-19, CAS-1 cells demonstrated that DPQ and PJ34 downregulate PARP-1 expression; they also cause a decrease of LDH release and nitrite production, while increasing cell viability. Similar effects were caused in all three cell lines also by N-mono-methyl-arginine (NMMA), a well known iNOS inhibitor, and by L-carnosine and trehalose, two antioxidant molecules. These results demonstrate that, similar to other well characterised drugs, DPQ and PJ34 reduce cell inflammation and damage ensuing PARP-1 overexpression, while they increase cell survival: this suggests their potential exploitation in clinical Medicine.

PARP-1 Inhibitors DPQ and PJ-34 Negatively Modulate Proinflammatory Commitment of Human Glioblastoma Cells.

SCALIA, Marina;SATRIANO, Cristina;RIZZARELLI, Enrico;SPINA, Vittoria Rita Annamaria
2013

Abstract

Inhibitors of Poly(ADP-ribose) polymerases (PARPs) have been proposed as promising chemotherapeutic agents in inflammatory, degenerative, and neoplastic diseases. To further substantiate this proposal, we tested the biomolecular effects of PARP-1 inhibitors DPQ and PJ34 on human glioblastoma (GBM) cells, induced to a proinflammatory state with Lipopolysaccharide (LPS) and Interferon-γ (INF-γ). PARP-1 expression was evaluated by laser scanning confocal microscopy (LSM); nitrite production, LDH release, cell viability were also determined. LSM of A172, SNB-19, CAS-1 cells demonstrated that DPQ and PJ34 downregulate PARP-1 expression; they also cause a decrease of LDH release and nitrite production, while increasing cell viability. Similar effects were caused in all three cell lines also by N-mono-methyl-arginine (NMMA), a well known iNOS inhibitor, and by L-carnosine and trehalose, two antioxidant molecules. These results demonstrate that, similar to other well characterised drugs, DPQ and PJ34 reduce cell inflammation and damage ensuing PARP-1 overexpression, while they increase cell survival: this suggests their potential exploitation in clinical Medicine.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11769/13187
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