This study tests the potential for using Armadillo officinalis as a bioindicator of exposure to and activationof benzene metabolic pathways using an in vivo model.A. officinalis specimens collected in a natural reserve were divided into a control and three test groupsexposed to 2.00, 5.32 or 9.09 mg/m3 benzene for 24 h. Three independent tests were performed to assessmodel reproducibility. Animals were dissected to obtain three pooled tissue samples per group: hepatopancreas(HEP), other organs and tissues (OOT), and exoskeleton (EXO). Muconic acid (MA), S-phenylmercapturicacid (S-PMA), two human metabolites of benzene, and changes in mtDNA copy number, ahuman biomarker of benzene exposure, were determined in each sample; benzene was determined onlyin EXO. MA was measured by high-performance liquid chromatography (HPLC) with ultraviolet (UV)detection, S-PMA by triple quadrupole mass spectrometer liquid chromatography with electro sprayionization (LC-MS-ESI-TQD), mtDNA by real-time quantitative PCR and end-point PCR, and benzene byquadrupole mass spectrometer head-space gas chromatography (HSGC-MS).MA and S-PMA levels rose both in HEP and OOT; EXO exhibited increasing benzene concentrations;and mtDNA copy number rose in HEP but not in OOT samples. Overall, our findings demonstrate that A.officinalis is a sensitive bioindicator of air benzene exposure and show for the first time its ability toreproduce human metabolic dynamics.

Validation of Armadillo officinalis Dumèril, 1816 (Crustacea,Isopoda, Oniscidea) as a bioindicator: In vivo study of air benzene exposure

AGODI, ANTONELLA PAOLA;OLIVERI CONTI, GEA MARZIA;BARCHITTA, MARTINA;QUATTROCCHI, ANNALISA;LOMBARDO, BIANCA MARIA;MONTESANTO, Giuseppe;MESSINA, GIUSEPPINA;FIORE, MARIA;FERRANTE, Margherita
2015-01-01

Abstract

This study tests the potential for using Armadillo officinalis as a bioindicator of exposure to and activationof benzene metabolic pathways using an in vivo model.A. officinalis specimens collected in a natural reserve were divided into a control and three test groupsexposed to 2.00, 5.32 or 9.09 mg/m3 benzene for 24 h. Three independent tests were performed to assessmodel reproducibility. Animals were dissected to obtain three pooled tissue samples per group: hepatopancreas(HEP), other organs and tissues (OOT), and exoskeleton (EXO). Muconic acid (MA), S-phenylmercapturicacid (S-PMA), two human metabolites of benzene, and changes in mtDNA copy number, ahuman biomarker of benzene exposure, were determined in each sample; benzene was determined onlyin EXO. MA was measured by high-performance liquid chromatography (HPLC) with ultraviolet (UV)detection, S-PMA by triple quadrupole mass spectrometer liquid chromatography with electro sprayionization (LC-MS-ESI-TQD), mtDNA by real-time quantitative PCR and end-point PCR, and benzene byquadrupole mass spectrometer head-space gas chromatography (HSGC-MS).MA and S-PMA levels rose both in HEP and OOT; EXO exhibited increasing benzene concentrations;and mtDNA copy number rose in HEP but not in OOT samples. Overall, our findings demonstrate that A.officinalis is a sensitive bioindicator of air benzene exposure and show for the first time its ability toreproduce human metabolic dynamics.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/15335
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