The immunoexpression profile of matrix metalloproteinase-13 was investigated for the first time in dentin of human caries and healthy teeth. Twelve permanent premolars (10 caries and 2 sound) were decalcified in ethylenediaminetetraacetic acid and proces - sed for embedding in paraffin wax. Sections 3- 4 μm in thickness were cut and processed for immunohistochemistry. A mouse monoclonal anti-metalloproteinase-13 antibody was used for localisation using an immunoperoxidase technique. Dentinal immunoreactivity was detected in all teeth; it was weak in sound teeth and strong close to the caries area. These in vivo findings suggest a role for metalloproteinase- 13 in the development and progression of adult human dental tissue disorders.
|Titolo:||Immunohistochemical analysis of matrix metalloproteinase-13 in human caries dentin.|
|Autori interni:||LORETO, CARLA AGATA|
LEONARDI, Rosalia Maria
|Data di pubblicazione:||2014|
|Rivista:||EUROPEAN JOURNAL OF HISTOCHEMISTRY|
|Appare nelle tipologie:||1.1 Articolo in rivista|