Two F1 progenies involving the cross of the globe artichoke genotype Romanesco C3 by the cultivated cardoon 'Altilis 41' as well as the wild cardoon 'Creta 4' were generated; the former allowed to construct the first cultivated cardoon map which was integrated with the one of globe artichoke. A wide set of SSR loci derived from ESTs was positioned into the reference genetic maps and a consensus SSR-based linkage map was developed. To further saturate the C. cardunculus map, with the goal to investigate the genetic basis controlling traits of interest, a broad-based sequencing approach was applied for the development of a large and robust SNP dataset. Next-Generation sequencing (NGS) technologies were applied using two complementary approaches: (i) genomic RAD (Restriction-site Associated DNA) tag sequencing in combination with the Illumina platform; (ii) transcriptome sequencing, via 454 (Roche) and Illumina technologies. These SNPs represent a one-stop resource to produce a dense C. cardunculus genetic map via high-throughput genotyping technologies.
|Titolo:||Development of molecular genetic maps and massive SNP mining through ngs technology in Cynara cardunculus L.|
|Data di pubblicazione:||2013|
|Appare nelle tipologie:||1.1 Articolo in rivista|