Cytosolic PLA2 (cPLA2) and Ca2+-independent PLA2 (iPLA2) play a significant role in insulin β-cells secretion. Bacterial infections may be responsible of the onset of diabetes. The mechanism by which Staphylococcus aureus infection of INS-1 cells alters glucose-induced insulin secretion has been examined. After acute infection, insulin secretion and PLA2 activities significantly increased. Moreover, increased expressions of phospho-cPLA2, phospho-PKCα and phospho-ERK 1/2 were observed. Chronic infection causes a decrease in insulin release and a significant increase of iPLA2 and COX-2 protein expression. Moreover, insulin secretion in infected cells could be restored using specific siRNAs against iPLA2 isoform and specific COX-2 inhibitor. Bacterial infections may be responsible for the onset of diabetes. S. aureus infection of INS-1 cells alters glucose-induced insulin secretion. Acute infection increases expressions of phospho-cPLA2, -PKCα and -ERK 1/2. Insulin secretion in infected cells is restored by specific PLA2-siRNAs. Insulin secretion in infected cells is restored by a specific COX-2 inhibitor.
Role of cytosolic and calcium independent phospholipases A2 in insulin secretion impairment of INS-1E cells infected by S. aureus
SALMERI, Mario;SCALIA, Marina;FRITTITTA, Lucia;TOSCANO, Maria Antonietta;ANFUSO, CARMELINA DANIELA;LUPO, Gabriella
2015-01-01
Abstract
Cytosolic PLA2 (cPLA2) and Ca2+-independent PLA2 (iPLA2) play a significant role in insulin β-cells secretion. Bacterial infections may be responsible of the onset of diabetes. The mechanism by which Staphylococcus aureus infection of INS-1 cells alters glucose-induced insulin secretion has been examined. After acute infection, insulin secretion and PLA2 activities significantly increased. Moreover, increased expressions of phospho-cPLA2, phospho-PKCα and phospho-ERK 1/2 were observed. Chronic infection causes a decrease in insulin release and a significant increase of iPLA2 and COX-2 protein expression. Moreover, insulin secretion in infected cells could be restored using specific siRNAs against iPLA2 isoform and specific COX-2 inhibitor. Bacterial infections may be responsible for the onset of diabetes. S. aureus infection of INS-1 cells alters glucose-induced insulin secretion. Acute infection increases expressions of phospho-cPLA2, -PKCα and -ERK 1/2. Insulin secretion in infected cells is restored by specific PLA2-siRNAs. Insulin secretion in infected cells is restored by a specific COX-2 inhibitor.File | Dimensione | Formato | |
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