In this study we show the outstanding agreement between simulation and experimental data concerning the efficient stabilization effect by NaCl of Z conformation. We demonstrate by circular dichroism (CD) experiments that Na+ not only is able to induce a B to Z form transition in a short (GC)(3) alternated portion of a sequence having 17 basis, but also is the best stabilizer in comparison with other Z inducers used (spermine and NiCl2). This result was confirmed by free energy calculations. Chirality 27:773-778, 2015. (c) 2015 Wiley Periodicals, Inc.
In this study we show the outstanding agreement between simulation and experimental data concerning the efficient stabilization effect by NaCl of Z conformation. We demonstrate by circular dichroism (CD) experiments that Na+ not only is able to induce a B to Z form transition in a short (GC)(3) alternated portion of a sequence having 17 basis, but also is the best stabilizer in comparison with other Z inducers used (spermine and NiCl2). This result was confirmed by free energy calculations. Chirality 27:773-778, 2015. (c) 2015 Wiley Periodicals, Inc.
Effect of Different Z-Inducers on the Stabilization of Z Portion in BZ-DNA Sequence: Correlation Between Experimental and Simulation Data
D'URSO, ALESSANDRO;PURRELLO, Roberto;
2015-01-01
Abstract
In this study we show the outstanding agreement between simulation and experimental data concerning the efficient stabilization effect by NaCl of Z conformation. We demonstrate by circular dichroism (CD) experiments that Na+ not only is able to induce a B to Z form transition in a short (GC)(3) alternated portion of a sequence having 17 basis, but also is the best stabilizer in comparison with other Z inducers used (spermine and NiCl2). This result was confirmed by free energy calculations. Chirality 27:773-778, 2015. (c) 2015 Wiley Periodicals, Inc.| File | Dimensione | Formato | |
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