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EnglishWe studied in vitro the effects of Tiagabine on genomic DNA of cortical rat astrocytes. To evaluate DNA damage, we used a relatively simple technique called Single Cell Gel Electrophoresis or Comet assay. Tiagabine was dissolved in culture medium and added at concentration of 1, 10, 20 and 50 microg/ml on 12-day old cultured astrocytes. In presence of 1 and 10 microg/ml of Tiagabine, no DNA damage was observed after 48 h of treatment. A moderate DNA damage was instead observed for cells exposed to 20 microg/ml of antiepileptic drug. Finally, DNA fragmentation was more evident after treatment with 50 microg/ml of Tiagabine. We conclude that Tiagabine, at the usual recommended doses, does not appear to influence negatively the cortical rat astrocytes, inducing DNA fragmentation only at very high concentrations.
| Titolo: | Tiagabine treatment and DNA damage in rat astrocytes: an in vitro study by Comet assay |
| Autori interni: | |
| Data di pubblicazione: | 2001 |
| Rivista: | |
| Handle: | http://hdl.handle.net/20.500.11769/196 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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