The complete structure of the lipopolysaccharide isolated from the Gram-negative marine bacterium Alteromonas addita, type strain KMM 3600(T) = R10SW13(T) has been elucidated by means of a combined chemical approach and state-of-the-art NMR and MS analyses. Isolation and characterisation of the lipid A moiety and the core oligosaccharide were pursued separately after either acid or alkaline treatment of the hpopolysaccharide. The structure detected was identified as a novel, highly negatively charged, deep-rough lipopolysaccharide in which a trisaccharide subunit is connected to a typical lipid A glucosamine backbone. Within the core oligosaccharide, a phosphodiester bridge connects a glucose unit to a heptose residue. (C) Wiley-VCH Verlag GmbH & Co.
The outer membrane of the marine gram-negative bacterium Alteromonas addita is composed of a very short-chain lipopolysaccharide with a high negative charge density
GAROZZO, DOMENICO;
2007-01-01
Abstract
The complete structure of the lipopolysaccharide isolated from the Gram-negative marine bacterium Alteromonas addita, type strain KMM 3600(T) = R10SW13(T) has been elucidated by means of a combined chemical approach and state-of-the-art NMR and MS analyses. Isolation and characterisation of the lipid A moiety and the core oligosaccharide were pursued separately after either acid or alkaline treatment of the hpopolysaccharide. The structure detected was identified as a novel, highly negatively charged, deep-rough lipopolysaccharide in which a trisaccharide subunit is connected to a typical lipid A glucosamine backbone. Within the core oligosaccharide, a phosphodiester bridge connects a glucose unit to a heptose residue. (C) Wiley-VCH Verlag GmbH & Co.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
