The complete structure of the lipopolysaccharide isolated from the Gram-negative marine bacterium Alteromonas addita, type strain KMM 3600(T) = R10SW13(T) has been elucidated by means of a combined chemical approach and state-of-the-art NMR and MS analyses. Isolation and characterisation of the lipid A moiety and the core oligosaccharide were pursued separately after either acid or alkaline treatment of the hpopolysaccharide. The structure detected was identified as a novel, highly negatively charged, deep-rough lipopolysaccharide in which a trisaccharide subunit is connected to a typical lipid A glucosamine backbone. Within the core oligosaccharide, a phosphodiester bridge connects a glucose unit to a heptose residue. (C) Wiley-VCH Verlag GmbH & Co.

The outer membrane of the marine gram-negative bacterium Alteromonas addita is composed of a very short-chain lipopolysaccharide with a high negative charge density

GAROZZO, DOMENICO;
2007-01-01

Abstract

The complete structure of the lipopolysaccharide isolated from the Gram-negative marine bacterium Alteromonas addita, type strain KMM 3600(T) = R10SW13(T) has been elucidated by means of a combined chemical approach and state-of-the-art NMR and MS analyses. Isolation and characterisation of the lipid A moiety and the core oligosaccharide were pursued separately after either acid or alkaline treatment of the hpopolysaccharide. The structure detected was identified as a novel, highly negatively charged, deep-rough lipopolysaccharide in which a trisaccharide subunit is connected to a typical lipid A glucosamine backbone. Within the core oligosaccharide, a phosphodiester bridge connects a glucose unit to a heptose residue. (C) Wiley-VCH Verlag GmbH & Co.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/243849
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