AIM: To determine whether or not matrix metalloproteinase 13 (MMP-13) is present in periapical granulomas with and without epithelium.METHODOLOGY: Seventeen open periapical granulomas of pulpal origin (seven lesionswithout epithelium and 10 with proliferating epithelium) were fixed in formalinand then embedded in paraffin prior to being processed for immunohistochemicalanalysis. A monoclonal antibody against human MMP-13 was used to evaluate MMP-13 expression. Immunocomplexes were subsequently treated with the secondary antibodyand then detected by means of streptavidin peroxidase. Immunoreactivity wasvisualized by development with 3,3'-diaminobenzidine.RESULTS: An immunopositive cytoplasmatic reaction for MMP-13 was observed in all the specimens, although the immunostaining by anti-MMP-13 antibody washeterogeneous and its levels varied according to histopathological findings. Inperiapical lesions without epithelium MMP-13 immunolabelling was detected in afew fibroblast-like cells, and in some plasma cells within the granulomatoustissue. A clear upregulation of MMP-13 expression was detected in periapicallesions with epithelium, especially in small island and thin strands ofepithelium.CONCLUSIONS: The expression pattern of MMP-13 demonstrates that it is involved inthe conversion of a periapical granuloma with epithelium into a radicular cyst.This property is related to the ability of MMP-13 to influence not only themigration of epithelial cell but also the invasion of granulomatous tissue.

Collagenase 3 expression (MMp-13) is expressed in periapical lesions: an immunohistochemical study

LEONARDI R;CALTABIANO, ROSARIO;LORETO, CARLA AGATA
2005-01-01

Abstract

AIM: To determine whether or not matrix metalloproteinase 13 (MMP-13) is present in periapical granulomas with and without epithelium.METHODOLOGY: Seventeen open periapical granulomas of pulpal origin (seven lesionswithout epithelium and 10 with proliferating epithelium) were fixed in formalinand then embedded in paraffin prior to being processed for immunohistochemicalanalysis. A monoclonal antibody against human MMP-13 was used to evaluate MMP-13 expression. Immunocomplexes were subsequently treated with the secondary antibodyand then detected by means of streptavidin peroxidase. Immunoreactivity wasvisualized by development with 3,3'-diaminobenzidine.RESULTS: An immunopositive cytoplasmatic reaction for MMP-13 was observed in all the specimens, although the immunostaining by anti-MMP-13 antibody washeterogeneous and its levels varied according to histopathological findings. Inperiapical lesions without epithelium MMP-13 immunolabelling was detected in afew fibroblast-like cells, and in some plasma cells within the granulomatoustissue. A clear upregulation of MMP-13 expression was detected in periapicallesions with epithelium, especially in small island and thin strands ofepithelium.CONCLUSIONS: The expression pattern of MMP-13 demonstrates that it is involved inthe conversion of a periapical granuloma with epithelium into a radicular cyst.This property is related to the ability of MMP-13 to influence not only themigration of epithelial cell but also the invasion of granulomatous tissue.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/25132
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