Leakage kinetics of a fluorescent dye from the interior of a vesicle (or cell) into atl external quencher containing well-stirred medium depends on the number and size of ''defects'' plaguing the vesicle's surface. In this paper we theoretically prove that a careful investigation of leak-age kinetics at early, intermediate, and late stages of the process can provide qualitative information on both the width and average spacing among the defects.
Modeling diffusion-controlled permeation through a fractured surface: Possibility of estimating the average distance among the fractures by fluorescence quenching measurements
GRASSI, Antonio;RAUDINO, Antonio
1996-01-01
Abstract
Leakage kinetics of a fluorescent dye from the interior of a vesicle (or cell) into atl external quencher containing well-stirred medium depends on the number and size of ''defects'' plaguing the vesicle's surface. In this paper we theoretically prove that a careful investigation of leak-age kinetics at early, intermediate, and late stages of the process can provide qualitative information on both the width and average spacing among the defects.File in questo prodotto:
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