In this study we investigated the variation of glucosinolate profile and theamount of antioxidant compounds (total phenols, ascorbic acid and carotenoids)during germination from seed to sprout at 10°C, 20°C and 30°C in two species,broccoli (Brassica oleracea var. italica) and rocket (Eruca sativa L.). The percentage ofgermination varied in relation to the species and the growth temperature: it wasgreatest in rocket and the better results were obtained at 20°C. The glucosinolateprofile was different between broccoli and rocket, with glucoraphanin as the maincomponent in broccoli and glucoerucin in rocket. The temperature of germination hada significant influence on their total amount, with the maximum at 30°C. Broccolishowed the highest antioxidant response at 10°C and 20°C, while rocket at 10°C. Seedextracts showed the lowest response. Simple linear regression analysis has beenperformed and the highest correlation was found between the two antioxidant assays.A significant correlation was also found between the two antioxidant assays and thetotal phenol content. On the other hand, the glucosinolate content and the antioxidantindexes were not significantly correlated.
The Glucosinolates and Variation of Antioxidant Compounds in Seeds and Sprouts of Broccoli (Brassica oleracea L. var. italica) and Rocket (Eruca sativa L.) in Relation to Temperature and Germinative Stage
BRANCA, Ferdinando;TRIBULATO, ALESSANDRO;
2013-01-01
Abstract
In this study we investigated the variation of glucosinolate profile and theamount of antioxidant compounds (total phenols, ascorbic acid and carotenoids)during germination from seed to sprout at 10°C, 20°C and 30°C in two species,broccoli (Brassica oleracea var. italica) and rocket (Eruca sativa L.). The percentage ofgermination varied in relation to the species and the growth temperature: it wasgreatest in rocket and the better results were obtained at 20°C. The glucosinolateprofile was different between broccoli and rocket, with glucoraphanin as the maincomponent in broccoli and glucoerucin in rocket. The temperature of germination hada significant influence on their total amount, with the maximum at 30°C. Broccolishowed the highest antioxidant response at 10°C and 20°C, while rocket at 10°C. Seedextracts showed the lowest response. Simple linear regression analysis has beenperformed and the highest correlation was found between the two antioxidant assays.A significant correlation was also found between the two antioxidant assays and thetotal phenol content. On the other hand, the glucosinolate content and the antioxidantindexes were not significantly correlated.File | Dimensione | Formato | |
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