Nanoparticles made of a conjugate of poly(D,L-lactide-co-glycolide) with alendronate (PLGAeALE NPs), were prepared by emulsion/solvent evaporation technique. The conjugation yield, determined by MALDI TOF analysis, was 30e35%. PLGAeALE NPs size, evaluated by photon correlation spectroscopy, was 198.7 0.2 nm. Haemocompatibility studies using different concentrations of PLGAeALE NPs did not show any significant effect on haemolysis, leukocyte number, platelet activation, APTT and complement consumption, in comparison with blood incubated with phosphate buffered saline (PBS). A significant reduction of the prothrombin activity was demonstrated after incubation with 560 mg/ml of PLGAeALE NPs; a significant increase was observed at the highest dilutions. The viability of human umbilical vein endothelial cells and bone marrow stromal cells (BMSC), evaluated through the neutral red test, was not affected by PLGAeALE NPs. There were no significant differences in cell-associated alkaline phosphatase between BMSC incubated with PLGAeALE NP- and PBS-added media. These results demonstrated that PLGAeALE NPs had an acceptable degree of blood compatibility and were not cytotoxic; therefore, they may be considered suitable for intravenous administration.
|Titolo:||Biocompatibility of poly(D,L-lactide-co-glycolide) nanoparticles conjugated with alendronate|
|Data di pubblicazione:||2008|
|Appare nelle tipologie:||1.1 Articolo in rivista|