MicroRNA in follicular fluid: New molecular markers of oocyte quality in cancer patients

Objective: to investigate the expression profile of microRNA in cancer patients, before radio/ chemiotherapy, compared with age-matched healthy patients. Patient(s): patients with malignant disease, younger than 35 years, with a good ovarian reserve and who didnâ t receive any prior treatment within comparative studies with healthy, age-matched patients whose primary infertility is due to a male factor. Main Outcome Measure(s): fast real time PCR system to investigate the expression miRNA profiles. 2-CT method to calculate relative gene expression levels between the two groups of patients. Bioinformatic tools for miRNA target prediction and pathway analysis. Results: our ongoing research project aims to identify highly represented miRNA in follicular fluid of cancer patients compared with healthy group. Our results will improve knowledge of the different signature which have a strong effect on reproductive health.