This contribution reports a novel entirely photocontrolled nanoplatform consisting in a binary mixture of Pluronic copolymers self-assembling in core-shell micelles and co-entrapping two photoactivatable components: a benzoporphyrin photosensitizer for photodynamic therapy (PDT) and a coumarin photocaged chemotherapeutic Chlorambucil (CAB). The resulting supramolecular micellar assembly is ca. 30 nm in diameter with polydispersity index below 0.1, stable for more than 72 h and exhibits excellent preservation of the photochemical properties of the two photoresponsive components, despite they are confined within the same host nanocarrier. The appropriate regulation of the relative concentrations makes these components able to absorb the visible light in comparable amount, leading to the effective simultaneous photogeneration of singlet oxygen and photo-triggered decaging of CAB. This "on demand" release of cytotoxic combinations results in an amplified anticancer activity against MCF-7 human breast adenocarcinoma cells.

Light-Controlled Simultaneous "On Demand" Release of Cytotoxic Combinations for Bimodal Killing of Cancer Cells

Fraix A;FAILLA, MARIA CRISTINA;Cardile V;Graziano AC;Rescifina A;Sortino S
2018

Abstract

This contribution reports a novel entirely photocontrolled nanoplatform consisting in a binary mixture of Pluronic copolymers self-assembling in core-shell micelles and co-entrapping two photoactivatable components: a benzoporphyrin photosensitizer for photodynamic therapy (PDT) and a coumarin photocaged chemotherapeutic Chlorambucil (CAB). The resulting supramolecular micellar assembly is ca. 30 nm in diameter with polydispersity index below 0.1, stable for more than 72 h and exhibits excellent preservation of the photochemical properties of the two photoresponsive components, despite they are confined within the same host nanocarrier. The appropriate regulation of the relative concentrations makes these components able to absorb the visible light in comparable amount, leading to the effective simultaneous photogeneration of singlet oxygen and photo-triggered decaging of CAB. This "on demand" release of cytotoxic combinations results in an amplified anticancer activity against MCF-7 human breast adenocarcinoma cells.
Light; Micelles; Multimodal Therapy; photodynamic therapy
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/327726
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