Embryo kinetics analysis is an emerging tool forselecting embryo(s) for transfer. The aim of the presentstudy was to determine morphokinetic parameters easilyusable in the laboratory and predictive of embryo developmentand, most importantly, of embryo competence in producinga clinical pregnancy after day 5 transfer. A retrospective time-lapse monitoring analysis ofmorphokinetic parameters for 72 fully implanted embryos(group A) were compared to 106 non-implanted embryos(group B), and to 66 embryos with arrested development fromthe same pool of group A. All the embryos were from 78patients undergoing ICSI treatment and day 5 embryo transfers.A day 3 embryo will develop into a viable blastocyst ifthe following ranges of morphokinetic parameters are met: t1(between 18.4 h and 30.9 h post-ICSI), t2 (21.4–34.8 h), t4 (33.1–57.2 h), t7 (46.1–82.5 h), t8 (46.4–97.8 h), tC-tF (7.7–22.9 h) ands3 (0.7–30.8 h). On day 5 embryos with the highest probability toimplant are those with a cc3 between 9.7 h and 21 h.Morphokinetic parameters are helpful to makeappropriate decisions for the disposition of each embryo. It isrecommended that each laboratory should determine its ownranges of in vitro development (IVD-MKP) and implantation associated(IMP-MKP) morphokinetic parameters.

The use of morphokinetic parameters to select all embryos with full capacity to implant

TOMASELLI, Venera;
2013-01-01

Abstract

Embryo kinetics analysis is an emerging tool forselecting embryo(s) for transfer. The aim of the presentstudy was to determine morphokinetic parameters easilyusable in the laboratory and predictive of embryo developmentand, most importantly, of embryo competence in producinga clinical pregnancy after day 5 transfer. A retrospective time-lapse monitoring analysis ofmorphokinetic parameters for 72 fully implanted embryos(group A) were compared to 106 non-implanted embryos(group B), and to 66 embryos with arrested development fromthe same pool of group A. All the embryos were from 78patients undergoing ICSI treatment and day 5 embryo transfers.A day 3 embryo will develop into a viable blastocyst ifthe following ranges of morphokinetic parameters are met: t1(between 18.4 h and 30.9 h post-ICSI), t2 (21.4–34.8 h), t4 (33.1–57.2 h), t7 (46.1–82.5 h), t8 (46.4–97.8 h), tC-tF (7.7–22.9 h) ands3 (0.7–30.8 h). On day 5 embryos with the highest probability toimplant are those with a cc3 between 9.7 h and 21 h.Morphokinetic parameters are helpful to makeappropriate decisions for the disposition of each embryo. It isrecommended that each laboratory should determine its ownranges of in vitro development (IVD-MKP) and implantation associated(IMP-MKP) morphokinetic parameters.
2013
Embryo competence; Morphokinetic parameter; Time-lapse monitoring; ANOVA test; Kolmogorov-Smirnov non parametric test; Kruskall-Wallis non-parametric test
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/32813
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