Photoactivable platforms for nitric oxide delivery with fluorescence imaging

The multifaceted role nitric oxide (NO) plays in human physiology and pathophysiology has stimulated a massive interest on NO-releasing compounds for therapeutic purposes. A main issue associated with use of NO donors is the precise spatiotemporal control of the NO release, as its effects are strictly site- and dose-dependent. NO photochemical precursors permit surmounting this difficulty since triggering with light offers an exquisite control of location and timing of NO delivery. On the other hand, the combination of NO photodonors with fluorescent components remains an urgent need for image-guided phototherapeutic treatments based on the use of NO. Fluorescence techniques permit not only an easy tracking of the photoprecursor in a biological environment but also the real-time quantification of the NO photoreleased therein in a non-invasive fashion. In this Focus Review we seek to provide an overview of recent advances in photoactivable platforms developed in our and other laboratories which combine the photoregulated release of NO with fluorescent functionalities. We shall focus attention on NO photoreleasing systems exhibiting 1) persistent fluorescence and 2) photoactivable fluorescence signals, highlighting their logical design and potential developments for phototheranostics.