In the present study of fusion between lipid vesicles performed by thermomechanical analysis, isothermal volume variation has been shown to be a reliable tool to follow these kinetics without introducing perturbing probes. In fact, the fusion process is accompanied by bilayer strain release which causes an overall volume decrease of the fused vesicles. Volumetric variations induced by side processes, such as adhesion or ion binding onto the vesicle surface, were accounted for in our measurements. Moreover, by the same technique we followed segregation effects of the membrane lipid components in mixed vesicles. The systems examined were neutral and anionic phospholipids containing vesicles. The role of temperature, vesicle size, lipid composition as well as the influence of different cations were also investigated. © 1991 Società Italiana di Fisica.

Isothermal volume variations in lipid vesicle suspensions. A new evidence of intervesicle fusion kinetics

ZUCCARELLO, Felice;RAUDINO, Antonio;LA ROSA, Carmelo;GRASSO, DOMENICO MARIA
1991

Abstract

In the present study of fusion between lipid vesicles performed by thermomechanical analysis, isothermal volume variation has been shown to be a reliable tool to follow these kinetics without introducing perturbing probes. In fact, the fusion process is accompanied by bilayer strain release which causes an overall volume decrease of the fused vesicles. Volumetric variations induced by side processes, such as adhesion or ion binding onto the vesicle surface, were accounted for in our measurements. Moreover, by the same technique we followed segregation effects of the membrane lipid components in mixed vesicles. The systems examined were neutral and anionic phospholipids containing vesicles. The role of temperature, vesicle size, lipid composition as well as the influence of different cations were also investigated. © 1991 Società Italiana di Fisica.
Physics of bioenergetic processes; Physics and Astronomy (all); Ocean Engineering; Mechanical Engineering; Electrical and Electronic Engineering; Control and Systems Engineering; Engineering (miscellaneous); Nuclear Energy and Engineering; Computer Science Applications1707 Computer Vision and Pattern Recognition; Chemical Engineering (all)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11769/357861
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