To investigate the effects of gabapentin, a structural analog of g-amino butyric acid(GABA), on the inflammatory response of lipopolysaccharide (LPS)-stimulated rabbitcorneal cells (SIRC) and on endotoxin-induced uveitis (EIU) in rabbits. We investigated the LPS-induced expression of several inflammatory mediators, such as TNF-a, IL-1b,cPLA2, COX-2, and PGE2 in the SIRC cells with or without gabapentin treatment.Gabapentin treatment significantly (p < 0.05) attenuated cytokines production, cPLA2activation, COX-2 expression, and PGE2 levels in SIRC. EIU was induced by anintraocular injection of 0.1 mg of LPS in albino rabbit eye. After 7 and 24 h fromLPS injection clinical signs of ocular inflammation were examined by slit lamp with orwithout topical treatment of 0.5% gabapentin. Tears, aqueous, cornea, conjunctiva,and iris-ciliary body were collected and inflammatory biomarkers assessed. Topicaltreatment with gabapentin significantly (p < 0.05) reduced clinical signs and biomarkersof inflammation compared with the LPS group both at 7 and 24 h. In conclusion, theresults generated in the present study suggest that ophthalmic formulation based ongabapentin may be useful in the treatment of inflammatory conditions associated toocular pain such as uveitis, and that clinical studies to evaluate this possibility may be warranted
Gabapentin Attenuates Ocular Inflammation: In vitro and In vivo Studies.
ANFUSO, CARMELINA DANIELA;LUPO, Gabriella;DRAGO, Filippo;BUCOLO, CLAUDIO
2017-01-01
Abstract
To investigate the effects of gabapentin, a structural analog of g-amino butyric acid(GABA), on the inflammatory response of lipopolysaccharide (LPS)-stimulated rabbitcorneal cells (SIRC) and on endotoxin-induced uveitis (EIU) in rabbits. We investigated the LPS-induced expression of several inflammatory mediators, such as TNF-a, IL-1b,cPLA2, COX-2, and PGE2 in the SIRC cells with or without gabapentin treatment.Gabapentin treatment significantly (p < 0.05) attenuated cytokines production, cPLA2activation, COX-2 expression, and PGE2 levels in SIRC. EIU was induced by anintraocular injection of 0.1 mg of LPS in albino rabbit eye. After 7 and 24 h fromLPS injection clinical signs of ocular inflammation were examined by slit lamp with orwithout topical treatment of 0.5% gabapentin. Tears, aqueous, cornea, conjunctiva,and iris-ciliary body were collected and inflammatory biomarkers assessed. Topicaltreatment with gabapentin significantly (p < 0.05) reduced clinical signs and biomarkersof inflammation compared with the LPS group both at 7 and 24 h. In conclusion, theresults generated in the present study suggest that ophthalmic formulation based ongabapentin may be useful in the treatment of inflammatory conditions associated toocular pain such as uveitis, and that clinical studies to evaluate this possibility may be warrantedFile | Dimensione | Formato | |
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