A multibiomarkers approach was used in order to estimate and monitor marine pollution. Coris julis (Linneaus, 1758) was chosen as a sentinel organism, and the specimens were collected from three wellknown sites along the Ionic coast of Sicily: the protected marine area (P.M.A) ‘‘Cyclop’s Islands’’ of Acitrezza (CT), used as a control site, Riposto (CT), and the industrial site of Augusta (SR). Abiotic levels of contaminants were also detected. High levels of biotic and abiotic accumulation were found at the industrial site in which the presence of genotoxic and oxidative damage were also evidenced, measured by Micronuclei, Alkaline and Fpg-modiﬁed Comet assays. The protein expression analysis showed metallothioneins (MTs) as good tissue-speciﬁc markers of metal accumulation. Their levels were signiﬁcantly higher in muscle than in liver tissue for all the sampling sites, with a positive correlation among tissue levels and the degree of pollution at the sites. Conversely, heat shock proteins 70 (HSP70) expression was higher in Augusta and Riposto than in the control site, but no signiﬁcant difference was found between the examined tissues among all sites.
|Titolo:||Biochemical and bioaccumulation approaches for investigating marine pollution using Mediterranean rainbow wrasse, Coris julis (Linneaus 1798)|
|Data di pubblicazione:||2012|
|Appare nelle tipologie:||1.1 Articolo in rivista|