A multibiomarkers approach was used in order to estimate and monitor marine pollution. Coris julis(Linneaus, 1758) was chosen as a sentinel organism, and the specimens were collected from three wellknown sites along the Ionic coast of Sicily: the protected marine area (P.M.A) ‘‘Cyclop’s Islands’’ of Acitrezza (CT), used as a control site, Riposto (CT), and the industrial site of Augusta (SR). Abiotic levels of contaminants were also detected. High levels of biotic and abiotic accumulation were found at theindustrial site in which the presence of genotoxic and oxidative damage were also evidenced, measured by Micronuclei, Alkaline and Fpg-modified Comet assays. The protein expression analysis showedmetallothioneins (MTs) as good tissue-specific markers of metal accumulation. Their levels weresignificantly higher in muscle than in liver tissue for all the sampling sites, with a positive correlation among tissue levels and the degree of pollution at the sites. Conversely, heat shock proteins 70 (HSP70)expression was higher in Augusta and Riposto than in the control site, but no significant difference was found between the examined tissues among all sites.
Biochemical and bioaccumulation approaches for investigating marine pollution using Mediterranean rainbow wrasse, Coris julis (Linneaus 1798)
TOMASELLO, BARBARAPrimo
;Copat CSecondo
Writing – Original Draft Preparation
;FERRITO, Venera;FERRANTE, Margherita;RENIS, Marcella;
2012-01-01
Abstract
A multibiomarkers approach was used in order to estimate and monitor marine pollution. Coris julis(Linneaus, 1758) was chosen as a sentinel organism, and the specimens were collected from three wellknown sites along the Ionic coast of Sicily: the protected marine area (P.M.A) ‘‘Cyclop’s Islands’’ of Acitrezza (CT), used as a control site, Riposto (CT), and the industrial site of Augusta (SR). Abiotic levels of contaminants were also detected. High levels of biotic and abiotic accumulation were found at theindustrial site in which the presence of genotoxic and oxidative damage were also evidenced, measured by Micronuclei, Alkaline and Fpg-modified Comet assays. The protein expression analysis showedmetallothioneins (MTs) as good tissue-specific markers of metal accumulation. Their levels weresignificantly higher in muscle than in liver tissue for all the sampling sites, with a positive correlation among tissue levels and the degree of pollution at the sites. Conversely, heat shock proteins 70 (HSP70)expression was higher in Augusta and Riposto than in the control site, but no significant difference was found between the examined tissues among all sites.File | Dimensione | Formato | |
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