We studied absorption, emission and lifetime of the coordination compound tris(2,2′-bipyridyl)ruthenium(II) fluorophore (Ru(bpy)3 2+) both dissolved in water solutions and dried. Lifetime measurements were carried out using a new detector, the Silicon Photomultiplier (SiPM), which is more sensitive and physically much smaller than conventional optical detectors, such as imager and scanner. Through these analyses and a morphological characterization with transmission electron microscopy, revealed its usability for sensor applications, in particular, as dye in optical DNA-chip technology, a viable alternative to the conventional CY5 fluorophore. The use of Ru(bpy)3 2+ would solve some of the typical disadvantages related to Cy5’s application, such as self-absorption of fluorescence and photobleaching. In addition, the Ru(bpy)3 2+ longer lifetime may play a key role in the definition of new optical DNA-chip.

Photo-physical characterization of fluorophore Ru(bpy)3 2+ for optical biosensing applications

Sinatra F;
2015

Abstract

We studied absorption, emission and lifetime of the coordination compound tris(2,2′-bipyridyl)ruthenium(II) fluorophore (Ru(bpy)3 2+) both dissolved in water solutions and dried. Lifetime measurements were carried out using a new detector, the Silicon Photomultiplier (SiPM), which is more sensitive and physically much smaller than conventional optical detectors, such as imager and scanner. Through these analyses and a morphological characterization with transmission electron microscopy, revealed its usability for sensor applications, in particular, as dye in optical DNA-chip technology, a viable alternative to the conventional CY5 fluorophore. The use of Ru(bpy)3 2+ would solve some of the typical disadvantages related to Cy5’s application, such as self-absorption of fluorescence and photobleaching. In addition, the Ru(bpy)3 2+ longer lifetime may play a key role in the definition of new optical DNA-chip.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/20.500.11769/36087
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