Effects of mycorrhizal inoculation and digestate fertilisation on triticale biomass production using fungicide-coated seeds

Crop fertilisation management using organic wastes and arbuscular mycorrhizal fungi (AMF) inoculation can play a crucial role in the sustainability of agroecosystems. However, in conventional agricultural systems, agrochemicals like fungicides could reduce the positive effect of AMF. The aim of this study was to evaluate the agronomic (biomass production) and environmental (soil CO 2 emission) effects of AMF inoculation and digestate spreading on triticale cultivation using commercial seeds coated with fungicide. The field experiment was conducted in 2014–2015 at the University of Padua’s experimental farm (Italy), adopting a split-plot design, where the main plot factor was AMF inoculation (inoculated vs. uninoculated) and the subplot factor was fertilisation treatment (no fertilisation (NF), digestate liquid fraction (DL), digestate solid fraction (DS), mineral fertilisation (MF)). Low AMF root colonization was observed, likely due to the effect of fungicide; the only significant effect of AMF inoculation was a lower shoot density. Dry biomass production was significantly higher in the MF treatment (21.8 ± 1.04 Mg/ha) and lower in the NF treatment (14.5 ± 0.73 Mg/ha) compared to DS and DL treatments, which were not significantly different with an average yield of 17.2 ± 2.10 Mg/ha. During the cropping season, soil CO 2 emissions were not significantly affected by either AMF inoculation or fertilisation treatment. The median value of soil CO 2 emissions was 447.3 mg/m 2 per hour.