A new Cu(I) probe (OBEP-CS1), bearing an alkyl-pyridinium moiety to get complete water solubility, was synthesized. Fluorescence titrations support copper binding and high selectivity in a water solution whereas formation of aggregates was excluded by DOSY experiments. The detection capability for intracellular Cu(I), especially localized in mitochondria, was demonstrated in differentiated neuroblastoma cells by confocal microscopy.

A novel fully water-soluble Cu(I) probe for fluorescence live cell imaging

TOMASELLI, Gaetano;SATRIANO, Cristina;TRUSSO SFRAZZETTO, GIUSEPPE
2014-01-01

Abstract

A new Cu(I) probe (OBEP-CS1), bearing an alkyl-pyridinium moiety to get complete water solubility, was synthesized. Fluorescence titrations support copper binding and high selectivity in a water solution whereas formation of aggregates was excluded by DOSY experiments. The detection capability for intracellular Cu(I), especially localized in mitochondria, was demonstrated in differentiated neuroblastoma cells by confocal microscopy.
File in questo prodotto:
File Dimensione Formato  
54 2014 ChemComm Giuffrida.pdf

solo gestori archivio

Tipologia: Versione Editoriale (PDF)
Licenza: NON PUBBLICO - Accesso privato/ristretto
Dimensione 2.22 MB
Formato Adobe PDF
2.22 MB Adobe PDF   Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.11769/37034
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 55
  • ???jsp.display-item.citation.isi??? 53
social impact