We have isolated the Drosophila melanogaster gene encoding the mitochondrial acyl carrier protein (mtACP), a subunit of NADH:ubiquinone oxidoreduc- tase involved in de novo fatty acid synthesis in the mi- tochondrion. This gene expresses two distinct mature transcripts by alternative splicing, which encode mature polypeptides of 86 (mtACP1A) and 88 (mtACP1B) amino acids, respectively. Drosophila mtACP1 is 72% identical to mammalian mtACP, 47% identical to Arabidopsis thaliana mtACP, and 46% identical to Neurospora crassa mtACP. The most highly conserved region encompasses the site that binds pantetheine-4¢- phosphate in all known ACPs. Southern analysis of genomic DNA and in situ hybridization to salivary gland chromosomes indicate that a single gene (mtacp1), located at 61F6±8, encodes the two isoforms of D. me- lanogaster mtACP1. Sequence analysis revealed that the gene contains four exons and that exons IIIA and IIIB are alternatively spliced. A P-element-induced loss-of- function mutation in the mtacp1 gene causes lethality, indicating that the gene is essential for viability. Devel- opmental Northern analysis shows that mtacp1 is ex- pressed at higher levels during late embryogenesis, in the pupa and in the adult. RNA in situ hybridization on embryos indicates that the mtacp1 gene is highly ex- pressed in the tracheal system. Zygotic mtacp1 function is required for both male and female gametogenesis.
á á Alternative splicing á
|Titolo:||The Drosophila melanogaster gene for NADH:ubiquinone oxireductase acyl carrier protein: developmental expression analysis and evidence for alternatively spliced forms|
|Data di pubblicazione:||1999|
|Citazione:||The Drosophila melanogaster gene for NADH:ubiquinone oxireductase acyl carrier protein: developmental expression analysis and evidence for alternatively spliced forms / G. RAGONE; R. CAIZZI; R.MOSCHETTI; P.BARSANTI; DE PINTO V; C. CAGGESE. - In: MOLECULAR AND GENERAL GENETICS. - ISSN 0026-8925. - 261(1999), pp. 690-697.|
|Appare nelle tipologie:||1.1 Articolo in rivista|