A simple LC method has been developed and validated to determine trans-3,5,3',4',5'-pentamethoxystilbene (PMS) in rat plasma. Chromatographic separation was achieved through gradient delivery of acetonitrile and water (1.5 mL min(-1)) at 50 A degrees C. PMS was quantified using UV detection at 320 nm. The standard curve ranged from 15 to 1,500 ng mL(-1). The intra- and inter-day precisions, in terms of CV, were all less than 10% while the inter-day and intra-day bias ranged from -6.8 to 8.3%. The plasma PMS levels were monitored in Sprague-Dawley rats after drug administration. This simple LC method appears to be useful in the pharmacokinetic investigation of PMS.
LC Determination of trans-3,5,3',4',5'-Pentamethoxystilbene in Rat Plasma RID G-2581-2010
TRINGALI, Corrado;
2010-01-01
Abstract
A simple LC method has been developed and validated to determine trans-3,5,3',4',5'-pentamethoxystilbene (PMS) in rat plasma. Chromatographic separation was achieved through gradient delivery of acetonitrile and water (1.5 mL min(-1)) at 50 A degrees C. PMS was quantified using UV detection at 320 nm. The standard curve ranged from 15 to 1,500 ng mL(-1). The intra- and inter-day precisions, in terms of CV, were all less than 10% while the inter-day and intra-day bias ranged from -6.8 to 8.3%. The plasma PMS levels were monitored in Sprague-Dawley rats after drug administration. This simple LC method appears to be useful in the pharmacokinetic investigation of PMS.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
